基于多基因序列和形态性状的牡丹组种间关系

牡丹被认为是中国的国花,具有很高的医学、观赏和经济价值.野生牡丹被认为是栽培牡丹的野生祖先,因此弄清牡丹组的种间亲缘关系具有重要的理论和实践意义.由于受到信息量的限制,根据单基凼数据或形态数据往往无法对牡丹组的种间关系得到明确的结果.本研究用12份样品代表野生牡丹组(Paeonia section Moutan DC.,Paeoniaceae)8个种,利用包括核基因(Adh1A、Adh2和GPAT)和叶绿体基因(trnS-trnG和rps16-trnQ)的DNA序列以及形态性状的多套数据来探讨野牛牡丹的种间关系.合并分析得到具高支持率的牡丹组物种间的系统发育关系.结果表明,芍药属牡丹组8个野生种分为两个亚组,即肉质花盘亚组subseet.Delavayanae和革质花盘亚组subsect.Vaginatae.肉质花盘亚组包括滇牡丹P delavayi和大花黄牡丹P.ludlowii;革质花盘亚组包括其余6个种.革质花盘亚组中,四川牡丹P.decomposita ssp.decomposita和紫斑牡丹P. rockii ssp. rockii关系密切;卵叶牡丹P.qiui和矮牡丹P. jishanenMs关系密切;银屏牡丹P. suffruticosa ssp.yinpingmudan与风丹P. ostii关系 密切,并且后两个分支为姊妹群.

Phylogenetic analysis of Paeonia sect.Moutan (Paeoniaceae) based on multiple DNA fragments and morphological data

Tree peony,being crowned the title"King of Flowers"in China,iS of great medicinal,ornamental,and economic values.In the present study.the phylogeny of the wild tree peony species (section Moutan,Paeonia, Paeoniaceae), represented by twelve accessions collected from all eight species in the section,was investigated based on the DNA sequence in five DNA fragments from both nuclear (Adhl A,Adh2 and GPAT)and chloroplast (trnS-trnG and rps16-trnQ) genomes.as well as morphological characters.Both maximum parsimony(MP)and Bayesian inference of phylogeny (BI) trees were reconstructed based on the combined data of the DNA sequences and morphological data,respectively.The MP and BI trees have the similar topology,and the sect.Moutan clearly branched into two clades.One clade consists of two species,P.delavayi and P ludlowii,corresponding to the subsect.Delavayanae.and another clade is composed of other six species.Within the second clade,the six species Can be divided into three subclades consisting of P.rockii and P.decomposita,P.jishanensis and P.qiui,P. suffruticosa and P ostii,respectively.Among the three subclades,P. jishanensis/P.qiui is most closely related to P.suffruticosa/P.ostii.These results provide up to date the clearest picture of the phylogeny of wild tree peony species in the sect.Moutan.