| 黄芩苷干预甲型H1N1流感病毒感染诱导的A549细胞周期分布及凋亡 |
| |
| 引用本文： | 张春晶,顾立刚,于海涛.黄芩苷干预甲型H1N1流感病毒感染诱导的A549细胞周期分布及凋亡[J].病毒学报,2011,27(2):108-116. |
| |
| 作者姓名： | 张春晶 顾立刚 于海涛 |
| |
| 作者单位： | 齐齐哈尔医学院生物化学与分子生物学生物遗传教研室,齐齐哈尔市,161042;北京中医药大学,中医药防治病毒性疾病教育部重点实验室,北京,100029 |
| |
| 基金项目： | 黑龙江省自然科学基金(No.D201027和QC2009C89); 教育部科学技术研究重点项目; 黑龙江省高等学校骨干教师资助计划资助~~ |
| |
| 摘 要： | 以甲型H1N1流感病毒作为刺激因素,在人肺腺癌A549细胞培养内采用MTT比色法和细胞病变(CPE)法观察黄芩苷不同作用时间的抗病毒效率;以碘化丙啶(Propidium iodide,PI)单染流式细胞仪分析细胞周期中各时期的细胞百分数和对细胞增殖的影响,以Hoechst33258染色荧光显微镜观察细胞凋亡的形态学变化,并采用免疫荧光实验测定膜受体通路Caspase 8和线粒体通路Caspase 9及作为细胞凋亡标志的Caspase 3的活性。结果显示:流感病毒感染36h后宿主细胞周期阻滞于S期(P<0.05),并在G0期细胞峰前出现一个亚二倍体细胞峰(凋亡细胞峰)。A549细胞中Caspase 8/3活性明显升高,但标记Caspase 9活性的荧光亮度增强不明显。黄芩苷对甲型流感病毒感染诱导的细胞损伤有较好的保护作用,最大剂量的黄芩苷100μg/mL无毒,可抑制病毒细胞病变的产生,50~100μg/mL治疗组可明显调节流感病毒感染诱导的细胞周期分布(P<0.05),细胞凋亡现象明显减少,100μg/mL黄芩苷治疗组Caspase 8/3活性显著降低,接近正常对照组细胞水平,有剂量依赖性。实验说明:黄芩苷可拮抗甲型流感病毒H1N1细胞病变,调控细胞周期分布,并通过抑制Caspase 8的激活,进一步抑制Caspase 3活性,从而发挥抗病毒作用。
|
| 关 键 词： | 黄芩苷 甲型流感病毒 细胞增殖 细胞凋亡 |
Antagonism of Baicalin on Cell Cyclical Distribution and Cell Apoptosis in A549 Cells Infected with Influenza A(H1N1) Virus |
| |
| ZHANG Chun-jing,GU Li-gang,YU Hai-tao.Antagonism of Baicalin on Cell Cyclical Distribution and Cell Apoptosis in A549 Cells Infected with Influenza A(H1N1) Virus[J].Chinese Journal of Virology,2011,27(2):108-116. |
| |
| Authors: | ZHANG Chun-jing GU Li-gang YU Hai-tao |
| |
| Institution: | ZHANG Chun-jing1,GU Li-gang2,YU Hai-tao1(1.Department of Biochemistry and Molecular Biology,Department of Cell Biology and Biology Genetics,Qiqihar Medical College,Qiqihar 161042,China,2.Education Ministry Key Laboratory of Anti-virus of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China) |
| |
| Abstract: | To observe the inhibitive effect of Baicalin against influenza A H1N1 virus infection in epithelial cell line A549,the cell proliferation and cytotoxicity were assayed by MTT,the cell cycle and the apoptosis were analyzed by flowcytometer using PI staining,the morphology of cellular nucleolus was observed by Hoechst 33258 staining and the effects of activation on caspase 3 and caspase 8/9 were also detected by immunofluorescent staining with a fluorescence microscope.The results showed that Baicalin exerted... |
| |
| Keywords: | baicalin type A influenza virus cell proliferation cell apoptosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
