Human amelogenin up-regulates osteogenic gene expression in human bone marrow stroma cells |
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| Authors: | Jingchao Hu Rong Shu Zhongchen Song Lan Chen |
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| Institution: | Department of Periodontology, Ninth People’s Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, No. 639 Zhi Zao Ju Road, Shanghai 200011, China |
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| Abstract: | Extracts of enamel matrix proteins are used to regenerate periodontal tissues. Amelogenin, the most abundant enamel protein, plays an important role in the regeneration of these tissues. However, the molecular mechanisms by which amelogenin contributes to periodontal regeneration remain unknown. Using primary human bone marrow stroma cells (hBMSCs) transduced with lentivirus encoding human amelogenin (hAm), we performed genome-wide expression profiling to analyze the effects of hAm transduction on the regulation of genes involved in osteogenic differentiation. Our results revealed that BMP-2, BMP-6, OPN and VEGFC were up-regulated. These results suggest that hAm may be a key element in regulating hBMSCs osteogenic differentiation. |
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| Keywords: | Abbreviations: EMPs enamel matrix proteins hAm human amelogenin hBMSCs human bone marrow stroma cells BMP bone morphogenetic protein OPN osteopontin VEGFC vascular endothelial growth factor C EGFP enhanced green fluorescent protein FCM flow cytometry rhBMP-2 recombinant human bone morphogenetic protein-2 TRAP tyrosine-rich amelogenin peptide |
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