Long term t in vitro storage of lily: effects of temperature and concentration of nutrients and sucrose |
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Authors: | FJM Bonnier JM Van Tuyl |
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Institution: | (1) Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Mansholtlaan 15, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands |
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Abstract: | Methods for long-term preservation of lily germplasm were examined. t In vitro regenerated bulblets of 10 lily (t Lilium L.)
genotypes (Asiatic hybrids, Oriental hybrids, t L. longiflorum and t L. henryi) were stored for 28 months at -2 °C and 25
°C on four different media: 1/4 or full strength Murashige and Skoog nutrients with 9% (w/v) or 6% sucrose. Sprout growth,
bulb growth, and viability were determined. The combination of 1/4 strength MS nutrients and 9% sucrose gave the highest reduction
in sprout and bulb growth, the highest viability and the highest percentage of regrowth after 28 months of storage. At 25
°C, all lily genotypes survived 28 months of storage under these conditions. At -2 °C, Asiatic and Oriental hybrids survived
28 months of storage, whereas genotypes of t L. longiflorum and t L. henryi survived 6 months of storage, but died during
prolonged storage.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | bulblet germplasm t Lilium minimal growth preservation |
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