Agrobacterium tumefaciens-mediated transformation to alter ethylene and cytokinin biosynthesis in broccoli |
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Authors: | Gapper Nigel E McKenzie Marian J Christey Mary C Braun Robert H Coupe Simon A Lill Ross E Jameson Paula E |
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Institution: | (1) New Zealand Institute for Crop & Food Research Limited, Private Bag 11600, Palmerston North, New Zealand;(2) Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand;(3) New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand |
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Abstract: | Broccoli (Brassica oleracea var. italica) deteriorates rapidly following harvest. Postharvest treatment of broccoli with 6-benzylaminopurine delays senescence, whilst exogenous ethylene has been shown to accelerate this process following harvest. To alter ethylene biosynthesis, broccoli was transformed, using Agrobacterium tumefaciens-mediated transformation, with an antisense ACC oxidase gene from broccoli driven by the asparagine synthetase promoter from asparagus. In addition, broccoli was transformed with the chimeric gene construct SAG12-IPT to alter cytokinin biosynthesis during harvest-induced senescence. Transformation was achieved using both hypocotyl and cotyledonary petiole explants. The presence of an antisense ACC oxidase gene enhanced transformation efficiency, but Ag+ incorporated into the medium did not. The transgenic nature of these plants was confirmed by PCR and Southern analyses. |
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Keywords: | Agrobacterium tumefaciens brassica cotyledonary petiole hypocotyl postharvest senescence transformation |
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