| 六角果鸢尾离体快繁技术体系的构建 |
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| 引用本文： | 戴长志,何平,张映雪,尹增芳.六角果鸢尾离体快繁技术体系的构建[J].热带亚热带植物学报,2018,26(4):415-420. |
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| 作者姓名： | 戴长志 何平 张映雪 尹增芳 |
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| 作者单位： | 南京林业大学南方现代林业协同创新中心生物与环境学院 |
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| 基金项目： | 江苏高校优势学科建设工程资助项目（PAPD）资助 |
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| 摘 要： | 为构建六角果鸢尾(Iris hexagona)的离体快繁体系,以其幼嫩根状茎为外植体,研究了培养基和植物生长调节剂对不定芽诱导、增殖和植株生根的影响。结果表明,根状茎用0.1%Hg Cl2消毒13 min的效果较佳;不定芽诱导最适培养基为MS+6-BA 1.5 mg L–1+NAA 0.5 mg L–1+蔗糖30 g L–1+琼脂7.5 g L–1,不定芽增殖的适宜培养基为MS+6-BA 0.5 mg L–1+NAA0.2 mg L–1+KT 0.3 mg L–1+蔗糖30 g L–1+琼脂7.5 g L–1;在MS+IBA 1.5 mg L–1+蔗糖30 g L–1+琼脂7.5 g L–1培养基上不定芽生根率可达100%;腐殖土和珍珠岩+泥炭土+蛭石(1∶2∶1)均可作为组培苗移栽的适宜基质,移栽成活率可达100%。
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| 关 键 词： | 六角果鸢尾 离体快繁 培养基 植物生长调节剂 |
| 收稿时间： | 2017/11/7 0:00:00 |
| 修稿时间： | 2018/2/6 0:00:00 |
Construction of Rapid Propagation System in vitro of Iris hexagona |
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| DAI Chang-zhi,HE Ping,ZHANG Yin-xue and YIN Zeng-fang.Construction of Rapid Propagation System in vitro of Iris hexagona[J].Journal of Tropical and Subtropical Botany,2018,26(4):415-420. |
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| Authors: | DAI Chang-zhi HE Ping ZHANG Yin-xue and YIN Zeng-fang |
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| Institution: | South Forestry Modern Forestry Collaborative Innovation Center, College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China,South Forestry Modern Forestry Collaborative Innovation Center, College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China,South Forestry Modern Forestry Collaborative Innovation Center, College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China and South Forestry Modern Forestry Collaborative Innovation Center, College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China |
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| Abstract: | |
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| Keywords: | Iris hexagona Rapid propagation in vitro Medium Plant growth regulator |
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