| 一个新葡萄糖淀粉酶基因的克隆与表达 |
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| 引用本文： | 杨立泉,戴小军,罗元明,马春晓,侯建华,武志强,王翠艳,李明刚.一个新葡萄糖淀粉酶基因的克隆与表达[J].生物工程学报,2007,23(3):477-482. |
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| 作者姓名： | 杨立泉 戴小军 罗元明 马春晓 侯建华 武志强 王翠艳 李明刚 |
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| 作者单位： | 1. 南开大学生物活性材料教育部重点实验室,天津300071
2. 中国科学院微生物研究所微生物资源前期开发国家重点实验室,北京,100080 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 根据已报道的米根霉葡萄糖淀粉酶基因序列,通过PCR方法,从天然少根根霉的总DNA中克隆到含有四个内含子的葡萄糖淀粉酶基因。通过设计引物并采取重叠PCR方法删除内含子,获得了新的少根根霉葡萄糖淀粉酶(Rhizopus arrhizu glucoamylase,RaGA)cDNA序列(Accession number:DQ903853)。该基因在毕赤酵母中成功表达,表达产物具有较高的葡萄糖淀粉酶活性。
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| 关 键 词： | 少根根霉 葡萄糖淀粉酶 克隆 表迭 毕赤酵母 |
| 文章编号： | 1000-3061（2007）03-0477-06 |
| 修稿时间： | 2006-11-032006-11-27 |
Cloning and Expression of a New Glucoamylase Gene |
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| YANG Li-Quan,DAI Xiao-Jun,LUO Yuan-Ming,MA Chun-Xiao,HOU Jian-Hu,WU Zhi-Qiang,WANG Cui-Yan and LI Ming-Gang.Cloning and Expression of a New Glucoamylase Gene[J].Chinese Journal of Biotechnology,2007,23(3):477-482. |
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| Authors: | YANG Li-Quan DAI Xiao-Jun LUO Yuan-Ming MA Chun-Xiao HOU Jian-Hu WU Zhi-Qiang WANG Cui-Yan and LI Ming-Gang |
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| Institution: | The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071,China;The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071,China;State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Science, Beijing 100080,china;The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071,China;The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071,China;The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071,China;The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071,China;The Key Laboratory of Bioactive Material, Ministry of Education, Life Science College, Nankai University, Tianjin 300071,China |
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| Abstract: | According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase. |
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| Keywords: | Rhizopus arrhizu glucoamylase clone expression Picha pastoris |
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