| 金色链霉菌DM-1全基因组序列测定及去甲金霉素合成基因簇分析 |
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| 引用本文： | 吴娜新,黄鹤,闵涛玲,胡海峰.金色链霉菌DM-1全基因组序列测定及去甲金霉素合成基因簇分析[J].生物工程学报,2020,36(12):2685-2694. |
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| 作者姓名： | 吴娜新 黄鹤 闵涛玲 胡海峰 |
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| 作者单位： | 1 中国医药工业研究总院 上海医药工业研究院，上海 201203;2 复旦大学 药学院，上海 201203;3 中国科学院 上海生命科学研究院 植物生理与生态研究所，上海 200032 |
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| 基金项目： | 国家重大新药创制课题 (No. 2014ZX09201-001-05)，上海市自然科学基金 (No. 15ZR1440300)，上海市产学研合作项目 (No. CXY-2013-53) 资助。 |
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| 摘 要： | 金色链霉菌Streptomyces aureofaciens DM-1是去甲基金霉素的高产菌株。通过Genome Sequencer FLX系统进行测序，得到一条完整的线性基因组序列，长度为6 824 334 bp，GC含量为72.6%。结合软件glimmer 3.02、Genemark和Z-Curve program进行基因预测，最终在其基因组中鉴定出6 431个基因。应用AntiSMASH软件预测其基因组中存在28个次级代谢生物合成基因簇，其中包含了去甲基金霉素生物合成基因簇。其中甲基转移酶CtcK因移码突变提前终止翻译，很可能是去甲基金霉素相对金霉素 (CTC) 缺失一个甲基的根本原因。研究结果为S. aureofaciens DM-1的功能基因组学和去甲基金霉素高产菌株育种提供了研究基础。
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| 关 键 词： | 金色链霉菌DM-1，基因组测序，去甲基金霉素，生物合成基因簇 |
| 收稿时间： | 2020/6/2 0:00:00 |
Genome sequencing of Streptomyces aureofaciens DM-1 and analysis of 6-demethylchlortetracycline biosynthesis gene cluster |
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| Naxin Wu,He Huang,Taoling Min,Haifeng Hu.Genome sequencing of Streptomyces aureofaciens DM-1 and analysis of 6-demethylchlortetracycline biosynthesis gene cluster[J].Chinese Journal of Biotechnology,2020,36(12):2685-2694. |
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| Authors: | Naxin Wu He Huang Taoling Min Haifeng Hu |
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| Institution: | 1 Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China;2 School of Pharmacy, Fudan University, Shanghai 201203, China;3 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China |
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| Abstract: | Streptomyces aureofaciens DM-1 is a high-yielding 6-demethylchlortetracycline producer. The genome sequencing of DM-1 reveals a linear chromosome containing 6 824 334 bps nucleotides with GC content of 72.6%. In this genome, a total of 6 431 open reading frames were predicted by using glimmer 3.02, Genemark and Z-Curve softwares. Twenty-eight secondary metabolite biosynthetic gene clusters were uncovered by using AntiSMASH gene prediction software, including the complete 6-demethylchlortetracycline biosynthetic gene cluster. A frame-shift mutation in methyltransferase coding region was detected, which may result in the demethylation of chlortetracycline. The complete genome sequence of S. aureofaciens DM-1 provides basic information for functional genomics studies and selection of high-yielding strains for 6-demethylchlortetracycline. |
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| Keywords: | Streptomyces aureofaciens DM-1 genome sequencing 6-demethylchlortetracycline biosynthetic gene cluster |
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