基因芯片技术研究柽柳NaHCO3胁迫下基因的表达

运用基因芯片技术研究了NaHCO3胁迫下柽柳(Tamarix androssowii)基因的表达.将Cy5和Cy3两种荧光染料分别标记在NaHCO3处理和对照的柽柳cDNA上,将两种荧光探针混合,与载有柽柳基因的高密度芯片进行杂交并用芯片扫描系统进行扫描,通过Cy5与Cy3信号强度比值的计算研究基因的差异表达.共获得了89个差异表达的基因,其中,27个下调表达,62个上调表达.BlastX分析表明这些基因按功能可以分为光合作用、活性氧清除、渗透调节、信号传导与表达调控、代谢、发育相关、核糖体蛋白、蛋白质的分解与再生、转运类蛋白、水通道蛋白等几大类别.同时,发现了一些与盐胁迫相关的功能未知基因或未有任何功能信息的基因,这些基因可能在柽柳抗盐过程中具有重要作用.揭示了柽柳的抗盐胁迫涉及的几种重要途径,并获得了NaHCO3胁迫前后柽柳基因表达谱.

Study on Expression of Genes in Tamarix androssowii under NaHCO3 Stress Using Gene Chip Technology

Gene chip technology was employed to study gene expression in Tamarix androsso wii under NaHCO 3 stress. cDNAs from T.androssowii treated with NaHCO 3 s olution and that from control group were labeled with fluorescent dye Cy5 and Cy 3 respectively. The two fluorescent cDNA probes were mixed and hybridized to gen e chips containing T.androssowii genes, and the chips were scanned using bio chip scanning system. Differential expression of genes was analyzed through calculat ion of the ratio of Cy5 to Cy3 signal intensities. Total of 89 genes differentia lly expressed were identified, among them, 27 showed down regulated expression a nd 62 showed up regulated expression. Blastx analysis showed that the function o f the differentially expressed genes could be grouped into some categorizations such as photosynthesis, reactive oxygen species eliminated, regulation of osmoti c potential, regulation of gene expression and signal transduction, metabolism, development, ribosomal protein, protein breakdown and recycling, transporter, wa ter channel proteins and so on. Based on this research, some function-unknown or novel unreported genes that respond to salt stress were also identified, and th ese genes may have important functions in salt resistance of T.androssowii. Some important pathways of salt resistance in T.androssowii are revealed, and th e ge ne expression profiling of T.androssowii under salt stress and without stres s is obtained in this study.