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珍贵用材树种红椿的组培育苗技术初探
引用本文:陈丽文,时群,梁刚,蔡林,何贵整.珍贵用材树种红椿的组培育苗技术初探[J].亚热带植物科学,2014(2):164-167.
作者姓名:陈丽文  时群  梁刚  蔡林  何贵整
作者单位:广西钦州市林业科学研究所;
基金项目:广西科学研究与技术开发计划项目(桂科攻10100012-8)
摘    要:以红椿种子为外植体材料,对其组织培养技术进行初步研究。结果表明,适宜红椿种子无菌苗芽诱导的培养基为MS+6-BA 0.5 mg·L^-1+NAA 0.2 mg·L^-1,适宜的芽继代增殖培养基为2/3MS+6-BA 0.5 mg·L^-1+NAA 0.2 mg·L^-1+GA 31.0 mg·L^-1,适宜生根培养基为1/2MS+IBA 0.5 mg·L^-1,以泥炭土、黄心土、河沙按1∶1∶1混合作为基质,移栽成活率达85%以上。

关 键 词:红椿  种子  组培育苗

Tissue Culture of Precious Timber Species Toona ciliata Roem
CHEN Li-wen,SHI Qun,LIANG Gang,CAI Lin,HE Gui-zheng.Tissue Culture of Precious Timber Species Toona ciliata Roem[J].Subtropical Plant Science,2014(2):164-167.
Authors:CHEN Li-wen  SHI Qun  LIANG Gang  CAI Lin  HE Gui-zheng
Institution:(Forestry Science Research Institute of Qinzhou City, Qinzhou 535099, Guangxi, China)
Abstract:The seeds of Toona ciliata were used as explants to study the tissue culture of T. ciliata. The results showed that the MS+6-BA 0.5 mg·L^-1+NAA 0.2 mg·L^-1 media was good for the bud induction of the asepsis seedling, the optimal bud proliferation culture medium was 2/3MS+6-BA 0.5 mg·L^-1+NAA 0.2 mg·L^-1+GA3 1.0 mg·L^-1, and the optimal root induction culture medium was 1/2MS+IBA 0.5 mg·L^-1, The plantlet was transplanted with the matrix of the peat soil, yellow subsoil and river sand, and the survival rates up to 85%.
Keywords:Toona ciliata  seed  tissue culture propagation
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