| 七种中草药提取物抗肿瘤活性部位的筛选研究初报 |
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| 引用本文： | 郑志忠,陈良华,郑国华,童宇星,张树峰,童庆宣,明艳林.七种中草药提取物抗肿瘤活性部位的筛选研究初报[J].亚热带植物科学,2011(3):31-35. |
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| 作者姓名： | 郑志忠 陈良华 郑国华 童宇星 张树峰 童庆宣 明艳林 |
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| 作者单位： | 厦门华侨亚热带植物引种园;厦门市植物引种检疫与植物源产物重点实验室; |
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| 基金项目： | 厦门市科技创新项目(3502Z20101016、3502220031037); 福建省自然科学基金(2010D012)资助 |
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| 摘 要： | 选取叶下珠、珠子草、千里光、鸦胆子、使君子、冬青和猫爪藤等7种中草药进行提取和初步分离,筛选出有抗肿瘤效果的活性部位,并对其进行初步分析。采用MTT法,以7种中草药醇提物的不同分离部位分别对人肝癌、胃癌、卵巢癌等肿瘤细胞株进行体外增殖抑制作用研究,并以人正常肝细胞株为模型对照跟踪筛选活性部位。结果表明,从7种中草药醇提物中,均获得对肿瘤细胞株具有体外增殖抑制作用的化学分离部位,其中叶下珠和珠子草的乙酸乙酯层和正丁醇层的活性最显著,千里光的乙酸乙酯层、鸦胆子的正丁醇层、使君子和冬青的氯仿层活性次之,猫爪藤氯仿层和冬青的乙酸乙酯层活性较差,其他部位无活性。
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| 关 键 词： | 中草药 MTT法 筛选 抗肿瘤 活性部位 |
Preliminary Study on Screening Anti-Tumor Active Fraction of Extracts from Seven Chinese Herbal Medicines |
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| ZHENG Zhi-zhong,CHEN Liang-hua,ZHENG Guo-hua,TONG Yu-xing,ZhANG Shu-feng,TONG Qing-xuan,MING Yan-lin.Preliminary Study on Screening Anti-Tumor Active Fraction of Extracts from Seven Chinese Herbal Medicines[J].Subtropical Plant Science,2011(3):31-35. |
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| Authors: | ZHENG Zhi-zhong CHEN Liang-hua ZHENG Guo-hua TONG Yu-xing ZhANG Shu-feng TONG Qing-xuan MING Yan-lin |
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| Institution: | ZHENG Zhi-zhong,CHEN Liang-hua,ZHENG Guo-hua,TONG Yu-xing,ZhANG Shu-feng,TONG Qing-xuan,MING Yan-lin(The Research and Development Center for Medicine Plant and Plant Drugs,Xiamen Oversea Chinese Subtropical Plant Introduction Garden,Xiamen Botanical Plant Introduction,Quarantine and Plant Products Key Laboratory,Xiamen 361002,Fujian China) |
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| Abstract: | Though extracting and separating on seven Chinese herbal medicines including Phyllanthus urinaria Linn.,Phyllanthus niruri Linn.,Senecio scandens Buch.-Ham.ex D.Don,Brucea javanica(Linn.) Merr,Quisqualis indica Linn.,Ilex chinensis Sims and Macfadyena unguis-cati(Linn.)A.Gentry,the anti-tumor active fractions were screened,and their anti-tumor effects on different kinds of human tumor cell lines were preliminary analyzed.The proliferation inhibitory effects in vitro of alcohol extracts on liver cancer,gastr... |
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| Keywords: | Chinese herbal medicine MTT assay screening anti-tumor active fraction |
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