| 台湾金线莲增殖培养正交试验设计 |
| |
| 引用本文： | 魏翠华,谢宇,陈沁,秦建彬.台湾金线莲增殖培养正交试验设计[J].亚热带植物科学,2012(4):57-59. |
| |
| 作者姓名： | 魏翠华 谢宇 陈沁 秦建彬 |
| |
| 作者单位： | 福州市农业科学研究所 |
| |
| 基金项目： | 福建省福州市科技计划项目(2011-N-29) |
| |
| 摘 要： | 以台湾金线莲试管苗茎段为外植体，应用L9（3^4）正交试验设计优选不定芽增殖培养基，结果表明，基本培养基、6-BA、NAA、蔗糖对不定芽增殖培养均有极显著影响，其作用表现为：蔗糖〉基本培养基〉6-BA〉NAA，最适宜的增殖培养基为1／2MS＋6-BA3．0mg／L＋NAA0．6mg／L＋蔗糖10g／L。
|
| 关 键 词： | 台湾金线莲 增殖培养 正交设计 |
Orthogonal Design on Proliferation Culture of Anoectochilus formosanus |
| |
| WEI Cui-hua,XIE Yu,CHEN Qin,QIN Jian-bin.Orthogonal Design on Proliferation Culture of Anoectochilus formosanus[J].Subtropical Plant Science,2012(4):57-59. |
| |
| Authors: | WEI Cui-hua XIE Yu CHEN Qin QIN Jian-bin |
| |
| Institution: | g(Fuzhou Institute of Agricultural Science,Fuzhou 350018,Fujian China) |
| |
| Abstract: | The optimization of culture medium for adventitious buds proliferation of Anoectochilus formosanus were studied by orthogonal design L9（34）, using the stems of test-tube seedling as explants. The results showed that the basic medium, 6-BA, NAA, sucrose had very significant effect on adventitious bud proliferation culture. The effect for proliferation culture was in the order of sucrose 〉 the basic medium 〉 6-BA 〉 NAA, the optimizing medium for proliferation culture was 1/2MS ＋ 6-BA 3.0 mg/L ＋ NAA 0.6 mg/L ＋ sucrose 10 g/L. |
| |
| Keywords: | Anoectochilusformosanus proliferation orthogonal design |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
