| 几种培养基及光照对蝴蝶兰叶片外植体褐变的影响 |
| |
| 引用本文： | 许传俊,李玲.几种培养基及光照对蝴蝶兰叶片外植体褐变的影响[J].亚热带植物科学,2006,35(1):9-12. |
| |
| 作者姓名： | 许传俊 李玲 |
| |
| 作者单位： | 1. 华南师范大学 生命科学学院, 广东省植物发育生物工程重点实验室, 广东 广州 510631;
2. 福建省亚热带植物研究所, 福建 厦门 361006 |
| |
| 基金项目： | 广东省博士启动基金;广东省科技厅科技计划 |
| |
| 摘 要： | 蝴蝶兰叶片外植体在MS纸桥培养基上培养10 d发生褐变率较低,1/2 MS次之,MS、B5和N6培养基外植体褐变率最高。MS培养基中铁盐加倍或微量元素缺乏造成外植体严重褐变,培养基积累很多褐色物质;减少琼脂含量,外植体褐变加重;添加柠檬酸可减轻外植体褐变;而添加活性炭、抗坏血酸或PVP对减轻外植体褐变无显著作用。光照强度增加外植体褐变严重。
|
| 关 键 词： | 蝴蝶兰 外植体 褐变 培养基 光照 |
| 文章编号： | 1009-7791（2006）01-0009-04 |
| 收稿时间： | 2005-09-02 |
| 修稿时间： | 2005-09-02 |
Influence of Medium and Light Intensity on the Leaf Explant Browning in Phalaenopsis sp. in vitro |
| |
| XU Chuan-jun,LI Ling.Influence of Medium and Light Intensity on the Leaf Explant Browning in Phalaenopsis sp. in vitro[J].Subtropical Plant Science,2006,35(1):9-12. |
| |
| Authors: | XU Chuan-jun LI Ling |
| |
| Institution: | 1. College of Life Science, South China Normal University, Guangdong Provincial Key Lab of Biotechnology for Plant Development, Guangzhou 510631, Guangdong China;
2. Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China |
| |
| Abstract: | The browning ratio ofPhalaenopsis sp. leaf explant in vitro growing on MS liquid paper bridge and 1/2MS after 10d is 0 and 67%, respectively, less than that growing on MS, B5 and N6, Doubling the content of FeSO4 or microelement deficiency could increase the browning rate of explant cultured in MS medium. Reducing the content of agar could increase the degree of browning The higher the intensity of light, the more serious the explant browning. Explant cultured on medium supplemented with citric acid showed a considerable reduction in browning. Adding Vit C, active carbon or PVP could not overcome the browning of explant. |
| |
| Keywords: | phalaenopsis sp explant browning medium light |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《亚热带植物科学》浏览原始摘要信息 |
| 点击此处可从《亚热带植物科学》下载免费的PDF全文 |
|
