| 发根农杆菌菌株的综合鉴定及活力比较 |
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| 引用本文： | 陶均,李玲.发根农杆菌菌株的综合鉴定及活力比较[J].亚热带植物科学,2005,34(2):11-14. |
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| 作者姓名： | 陶均 李玲 |
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| 作者单位： | 华南师范大学,生命科学学院,广东省植物发育生物工程重点实验室,广东,广州,510631 |
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| 基金项目： | 广东省自然科学基金项目(003062)资助 |
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| 摘 要： | 利用3-酮乳糖产物法、差异酸生成实验和游动性实验鉴定发根农杆菌菌株A4、R1205、R1000、R1601、R1022和15834的菌株类型和活力。结果表明,R1205、R1601、R1000、A4为Ⅱ型农杆菌,其活力从大到小依次是R1000、R1205、A4、R1601。利用PCR方法鉴定表明,A4、R1205、R1000和R1601为发根农杆菌,而R1022和15834未出现阳性结果。黄瓜遗传转化力鉴定结果表明,R1000的遗传转化力最大,达到79.16%,其它菌株依次是R1205、R1601、A4。根据上述三方面综合鉴定,R1000菌株活力最大。
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| 关 键 词： | 发根农杆菌 鉴定 菌株活力 |
| 文章编号： | 1009-7791(2005)02-0011-04 |
| 修稿时间： | 2004年12月29 |
Comprehensive Identification and Vigor Comparison of Agrobacterium rhizogenes |
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| TAO Jun,LI Ling.Comprehensive Identification and Vigor Comparison of Agrobacterium rhizogenes[J].Subtropical Plant Science,2005,34(2):11-14. |
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| Authors: | TAO Jun LI Ling |
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| Abstract: | The comparison and identification of Agrobacterium strains (A4,R1205, R1000, R1601, R1022 and 15834) were investigated by 3-ketolactose production, differential acid production and motility test. The result indicated that R1205, R1601, R1000and A4 belonged to biotype II and the vigor of strains was R1000>R1205>A4>R1601. According to PCR assay, four strains (A4, R1205, R1000, R1601) were Agrobacterium rhizogenes. According to the transformatic model cucumber, which mediated by A4, R1205, R1000 and R1601, the transformatic frequency of R1000 was the highest (79.6%), and followed by R1205, A4 and R1601. Based on the experiment results, the vigor of R1000 was the highest. |
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| Keywords: | Agrobacterium rhizogenes identification vigor |
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