柠檬醛损伤黄曲霉线粒体生化机理的研究

应用生物化学方法并结合扫描电镜，研究柠檬醛掺入黄曲霉细胞，并通过损伤线粒体(Mt)，导致抑制其生长的机理。结果表明，在药物致敏浓度时，菌丝体经该醛作用后，胞内Mt呈不规则增多，氧化还原反应系统受到破坏，与对照组相比，柠檬醛组的琥珀酸脱氢酶(Succinate Dehydrogenase,SDH)、苹果酸脱氢酶(Malate Dehydrogenase,MDH)活性分别呈不可逆下降271%和242%，随着药物浓度的升高，SDH、MDH的活性直至消失；以琥珀酸、α酮戊二酸和丙酮酸为底物时，线粒体呼吸速率分别下降24.1%、14.3%和36.1%，提示柠檬醛能使菌丝体DNA、RNA、脂类和蛋白质等生物合成受到抑制，促进细胞死亡。

Study on Biochemical Mechanism of Citral Damage to the A.flavasi's Mitochondria

Adopted biochemical method and combinded with Scanning Electron Microscope(SEM) to observe, study the inhibition-growth mechanism to A. flavus when citral was permeated to the cell and damaged mitochondria, the result suggested that the mitochondria changed in many kinds of unrule shape and the oxidation-reduction system was destroyed, when the citral reached at the level of anaphylactic consistency to A. flavus. Compared with the control group, activities of MDH and SDH in experimental group non-reversibly decreased 27.1% and 23.8% respectively, and grandully lost. When respectively used the succinate, pyruvate and alpha-ketoglutarate as substrates, the respiration speed of mitochondria separatelly decreased 24.1%, 36.1%, 14.3%. It suggested that the citral could inhibit the biosynthesis of the mycelial DNA, RNA, lipoid and protein, so promoted to dath.