| 香石竹设施栽培根际土壤微生物区系的16S rRNA系统遗传多样性 |
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| 引用本文： | 高旭红,何松涛,官会林,唐蜀昆,李文均,陈晓波.香石竹设施栽培根际土壤微生物区系的16S rRNA系统遗传多样性[J].微生物学报,2011,51(6):811-818. |
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| 作者姓名： | 高旭红 何松涛 官会林 唐蜀昆 李文均 陈晓波 |
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| 作者单位： | 1. 云南师范大学能源与环境科学学院,昆明,650092
2. 云南大学云南省微生物研究所,昆明,650091 |
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| 基金项目： | 国家自然科学基金(40861019）; 国家水体污染控制与治理科技重大专项（2009ZX07102－004） |
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| 摘 要： | 【目的】针对香石竹设施栽培土传病害的生物防治技术研究,探讨其根际土壤微生物与枯萎病害的关联性。【方法】采集香石竹健康植株与枯萎病植株根际土壤,采用不同培养基进行分离、纯化,并对分离菌株提取基因组DNA,用其16S rRNA序列的通用引物进行PCR扩增,进行blast同源分析。【结果】从采集样品中分离出的菌株分布于细菌域(Bacteria)中的4个门(Phyla)共15个属(Genera),其中从健康植株组土壤中培养出65株菌,分布于9个属,并以芽孢杆菌(Bacillus)、链霉菌(Streptomyces)及孢霉菌(Mortierella)为优势菌群;而枯萎病株组土样共培养出33株菌,分布于12个属,并且寡养单胞菌(Stenotrophomonas)、鞘氨醇杆菌(Sphingobacterium)、假单胞菌(Pseudomonas)、金黄杆菌(Chryseobacterium)、拟无枝菌酸菌(Amycolatopsis)及尖镰孢病原菌(Fusarium oxysporum)属的分离菌株仅从病株组土壤中分离到;分离菌株同源性在90%-98%的潜在新种(potential novel species)有13株。【结论】研究结果表明,根际土壤中真菌数与总菌数的百分比或Bacillus类群多样性的丰度,可作为评价区域香石竹种植土壤健康状况、栽培土壤演变及病害防治预测预报的参考指标。
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| 关 键 词： | 香石竹 土壤微生物 枯萎病害 |
| 收稿时间： | 2010/12/5 0:00:00 |
| 修稿时间： | 2011/3/21 0:00:00 |
Distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse |
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| Xuhong Gao,Songtao He,Huilin Guan,Shukun Tang,Wenjun Li and Xiaobo Chen.Distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse[J].Acta Microbiologica Sinica,2011,51(6):811-818. |
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| Authors: | Xuhong Gao Songtao He Huilin Guan Shukun Tang Wenjun Li and Xiaobo Chen |
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| Institution: | Energy and Environment Science college, Yunnan Normal University, Kunming 650092, China;Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China';Energy and Environment Science college, Yunnan Normal University, Kunming 650092, China;Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China;Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China;Energy and Environment Science college, Yunnan Normal University, Kunming 650092, China |
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| Abstract: | Objective] To investigate the relationship between microbial community and the blight diseases in rhizosphere of carnation for biological control.Methods] Bacterial strains isolated from the rhizosphere of healthy and blight carnation plants in greenhouse were replicated by morphology and 16S rRNA gene similarity,and investigated by using phylogenetic analysis based on 16S rRNA gene sequence alignments.Results] Isolates belonged to 4 phyla of bacteria,65 isolates from the samples of healthy carnation pla... |
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| Keywords: | carnation soil microbes blight diseases |
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