| 杀蜚蠊毒素的研究 |
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| 引用本文： | 王春生,陈涛,夏克祥,李彦,刘勇,杨冬菊,尹明珍.杀蜚蠊毒素的研究[J].微生物学报,1992,32(5):383-386. |
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| 作者姓名： | 王春生 陈涛 夏克祥 李彦 刘勇 杨冬菊 尹明珍 |
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| 作者单位： | 中国科学院武汉病毒研究所 武汉 430071 |
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| 摘 要： |
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| 关 键 词： | 杀虫微生物 蜚蠊 毒素 |
Studies of cockroachcidal toxin] |
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| C Wang,T Chen,K Xia,Y Li,Y Liu,D Yang,M Yin.Studies of cockroachcidal toxin][J].Acta Microbiologica Sinica,1992,32(5):383-386. |
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| Authors: | C Wang T Chen K Xia Y Li Y Liu D Yang M Yin |
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| Institution: | Wuhan Institute of Virology, Academia Sinica. |
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| Abstract: | A cockroachcidal bacterial isolate CW-W-90-3 was selected by egg yolk agar plate. The isolate produced phospholipase C (PLC) which was pathogenic to the nymph of cockroach. The conditions for production of high level PLC indicated that using LB medium supplement Tween-80 or minimal medium could effectively increase the activity of PLC. The optical phase for production of PLC was in the period of 12-18 hours and below pH8.0. The activity of PLC was reduced along with the culture time until 48 hours. The PLC was resistant to heat. The partially purified PLC from the culture supernatant was assayed by using cockroach nymphes and produced 71.74% mortality. |
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