| 三条新颖短肽筛选及抑菌作用的初报 |
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| 引用本文： | 兰金苹,李莉云,汪洋,王宪云,刘丽娟,刘国振,成雄鹰.三条新颖短肽筛选及抑菌作用的初报[J].微生物学报,2011,51(12):1675-1680. |
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| 作者姓名： | 兰金苹 李莉云 汪洋 王宪云 刘丽娟 刘国振 成雄鹰 |
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| 作者单位： | 1. 河北农业大学生命科学学院,保定,071001
2. Vitegen Limited Liability Company, Gary, NC27518, USA |
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| 基金项目： | 国家自然科学基金(30670175，30730007);国家“973 项目”(2007CB109201，2006CB101705) |
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| 摘 要： | 目的]为了发现新的农作物病原菌抗菌肽,人工设计并构建了大容量短肽文库,从中筛选并合成96条短肽用于鉴定其对农作物病原菌的抑菌活性.方法]采用琼脂扩散法,对靶标菌一棉花枯萎病菌(Fusarium f.sp.vasinfecum)、棉花红腐病菌(Fusarium moniliforme)、小麦根腐病菌(Bipolaris sorokiniana)和马铃薯早疫病菌(Alternaria solani)进行抑菌初筛,并测定了有抗菌作用短肽的最小抑菌浓度和抑菌持久性.结果]得到了A6、D4和F10对上述四种病原真菌抑菌效果较强,抑菌时间较长的抗菌肽,通过与抗菌肽数据库氨基酸序列对比,未见这3条抗菌肽的同源序列.结论]研制的3条短肽属于新颖抗菌肽,为防治农作物真菌病害提供了新的基因资源.
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| 关 键 词： | 新颖抗菌肽 筛选 病原真菌 抑菌作用 |
| 收稿时间： | 7/6/2011 12:00:00 AM |
| 修稿时间： | 2011/10/18 0:00:00 |
Screening of three novel antimicrobial peptides with antifungal pathogens |
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| Jinping Lan,Liyun Li,Yang Wang,Xianyun Wang,Lijuan Liu,Gouzhen Liu and Xiongying Cheng.Screening of three novel antimicrobial peptides with antifungal pathogens[J].Acta Microbiologica Sinica,2011,51(12):1675-1680. |
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| Authors: | Jinping Lan Liyun Li Yang Wang Xianyun Wang Lijuan Liu Gouzhen Liu and Xiongying Cheng |
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| Institution: | Jinping Lan1,Liyun Li1,Yang Wang1,Xianyun Wang1,Lijuan Liu1,Gouzhen Liu1,Xiongying Cheng2 1 College of Life Sciences,Agricultural University of Hebei,Baoding 071001,China 2 Vitegen Limited Liability Company,Cary,NC27518,USA |
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| Abstract: | Objective] In order to discover novel antimicrobial peptides against important crop pathogens,we designed and screened a high capacity random peptide library and isolated a number of clones expressing peptides with antifungal activity.We selected 96 peptides from the library and synthesized their sequence,which were used to assay their activity against crop fungal pathogens.Methods] Using agar diffusion assay,these peptides were assayed for their activity against pathogens that cause cotton Fusarium wilt(... |
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| Keywords: | novel antimicrobial peptides screening fungal pathogens anti-fungal activity |
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