| 用AFLP技术研究花生根瘤菌的遗传多样性 |
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| 引用本文： | 张小平 陈强 李登煜 Lindstrm K.用AFLP技术研究花生根瘤菌的遗传多样性[J].微生物学报,1999,39(6):483-488. |
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| 作者姓名： | 张小平 陈强 李登煜 Lindstrm K |
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| 作者单位： | 四川农业大学农学院微生物系 雅安 625000；芬兰赫尔辛基大学应用化学与微生物系 00014 |
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| 基金项目： | 国家自然科学基金，欧盟国际合作项目 |
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| 摘 要： | 采用AFLP分子标记技术，对分离自中国、津巴布韦、以色列的133株慢生花生根瘤菌(\%Bradyrhizobium \%sp.\%arachis)\%和13个代表菌株(\%Bradyrhizobium japonicum. Bradyrhizobium elkanii)\%的DNA扩增长度多态性进行了分析，并根据各供试菌株的遗传相似性进行了数值聚类。结果表明慢生花生根瘤菌群体内存在很高的遗传多样性，每个菌株的AFLP带谱均与其它菌株完全不同。AFLP技术简便、快速、重现性极高，能表现高信息量的DNA长度多态性，是目前研究生物群体内遗传多样性的最有效办法。
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| 关 键 词： | 花生根瘤菌， 多样性， AFLP |
THE USE OF AFLP TECHNIQUE FOR THE STUDY OF GENETIC DIVERSITY IN PEANUT BRADYRHIZOBIA |
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| X Zhang,Q Chen,D Li,K Lindstr?m.THE USE OF AFLP TECHNIQUE FOR THE STUDY OF GENETIC DIVERSITY IN PEANUT BRADYRHIZOBIA[J].Acta Microbiologica Sinica,1999,39(6):483-488. |
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| Authors: | X Zhang Q Chen D Li K Lindstr?m |
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| Institution: | Deparment of Microbiology, Sichuan Agriculture University, Yaan 625000. |
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| Abstract: | A total of 146 bradyrhizobial strains including 133 peanut bradyrhizobia isolated from different regions and 13 reference strains representing \% B. japonicum\% and \%B.elkanii\% were studied for their genetic diversity by use of AFLP marker technique. The genomic similarity was analysed by numerical Taxonomy based on the AFLP patterns. The results showed that the genetic diversity of peanut bradyrhizbia were very high. Each strain had a identical AFLP pattern. AFLP technique is simple, fast, highly reproducible, and is a useful tool for evaluating genetic diversity. |
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| Keywords: | Peanut bradyrhizobia Diversity AFLP |
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