用AFLP技术和16S rDNA PCR-RFLP分析毛苜蓿根瘤菌的遗传多样性

采用选择主增片断长度多态性（简称ＡＦＬＰ）ＤＮＡ指纹技术对采自我国云南省与西藏交界的高山地区的野生型豆科植物毛苜蓿根际土样分离的２９１株毛苜蓿（Ｍｅｄｉｃａｇｏ ｅｄｇｅｗｏｒｔｈｉｉ）根瘤菌进行遗传多样性的研究。从ＡＦＬＰ图谱中，揭示出毛苜蓿根瘤菌有较显著的遗传多样性，从２９１株中选择出９０年代表株用计算机进行树状图的分析。结果表明，所分析的菌株在７９％的相似性水平上聚类成３个群。对这９０年代表

GENETIC DIVERSITY OF RHIZOBIA OF MEDICAGO EDGEWORTHII BY AFLP AND RFLP ANALYSIS

Two hundred and ninety one isolates of rhizobia sampled from root nodules of \%Medicago edgeworthii\% which came from Yun Nan Province in china were analyzed by amplified restriction fragment polymorphism(AFLP) technique. According to the AFLP banding patterns, the results showed most of these isolates were genetically diversity. Ninety isolates were selected as representing the diversity among the 291 isolates. The 90 isolates were clustered into three groups at the level of similarity of 79% by computer analysis of the data. Additional representative isolates and reference strains were identified by restriction fragment length polymorphism (RFLP) analysis of PCR\|Amplified 16S rDNA. Two different 16S rDNA PCR\|RFLP types were found, which indicated that the isoltes were phylogentically closely related to \%Rhizobium mongolense.\%