| 微菌落观察法快速检测和鉴别结核杆菌培养物的研究 |
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| 引用本文： | 匡铁吉,宋萍,金关甫,王仲元,朱建华,刘金伟,秦振伦,李伟霞,陈洪斌.微菌落观察法快速检测和鉴别结核杆菌培养物的研究[J].微生物学报,1996,36(6):472-475. |
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| 作者姓名： | 匡铁吉 宋萍 金关甫 王仲元 朱建华 刘金伟 秦振伦 李伟霞 陈洪斌 |
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| 作者单位： | 解放军三○九医院 解放军三○九医院 北京 |
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| 摘 要： | 将取自肺结核患者的219例痰标本接种匡氏琼脂平板,共分离到112例结核菌生长物。其中培养法104例(92.8％)阳性,微菌落观察法108例(96.4％)阳性,微菌落法阳性率稍高。培养法和微菌落法阳性标本首次检出时间分别为18.6d和11d,微菌落检出时间更短(P<0.01)。常规菌型鉴别方法与微菌落法对结核杆菌菌型鉴别的符合率为99％。
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| 关 键 词： | 结核杆菌 微菌落 琼脂培养基 |
MICROCOLONY OBSERVATION FOR RAPID DETECTION AND IDENTIFICATION OF CULTURES OF MYCOBACTERIUM TUBERCULOSIS |
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| Kuang Tieji Song Ping Jin Guanfu Wang Zhongyuan Zhu Jianhua.MICROCOLONY OBSERVATION FOR RAPID DETECTION AND IDENTIFICATION OF CULTURES OF MYCOBACTERIUM TUBERCULOSIS[J].Acta Microbiologica Sinica,1996,36(6):472-475. |
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| Authors: | Kuang Tieji Song Ping Jin Guanfu Wang Zhongyuan Zhu Jianhua |
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| Abstract: | 219 sputa were seeded on Kuang's agar plates. A total of 112 isolates of Mycobacterium tuberculosis were detected in 219 specimens. Of these 112 isolates, 104 (92.8%) were detected in Kuang's agar medium arid 108(96.4%) were detected by microcolony observation. The detection time of microcolony observation and culture method needed 11 and 18.6 days respectively. The detection time of microcolony method is much shorter (P< 0.001). The results of conventional tests of different species of Mycobacterium and microcolony differentiation were identical in 99% of isolates of Mycobacterium tuberculosis. |
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| Keywords: | Mycobacterium tuberculosis Microcolony Agar medium |
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