一株新的溶磷棘孢青霉菌Z32的分离、鉴定及其土壤定殖与溶磷特性

【目的】从植物根部土壤中分离到一株高效溶磷真菌Z32,进行了分类学鉴定和土壤定殖与溶磷特性的初步研究。为溶磷微生物的应用提供新的菌株。【方法】通过形态特征、培养特征和ITSrDNA序列分析方法进行菌株鉴定。通过菌株Z32在土豆液体培养基培养过程中培养液pH的变化确定溶磷菌株的溶磷能力。利用菌株土培试验,进行菌株的土壤定殖和土壤中不同形态无机磷转化试验。【结果】菌株Z32鉴定为棘孢青霉菌(Penicillium aculeatum)。菌株Z32能在4d内完全溶解固体培养基中的磷酸三钙,18h内将土豆液体培养基的pH值从7.0降低到1.5左右。菌株Z32在20℃时的定殖效果最好,21d时,菌数达到起始的9.83倍,而且能够保持到49d不消亡。在20℃时,添加菌株Z32的土培实验在21d时,土壤中Ca8H2(PO4)6·5H2O、AlPO4和FePO4等难溶无机磷向可溶性的CaHPO4转化,CaHPO4含量增加了58.83%。49d时,土壤中的Ca8H2(PO4)6·5H2O、AlPO4和和FePO4被转化后,没有随着微生物的减少而完全被固定。【结论】筛选到一株新的溶磷菌株Z32在土壤中很好定殖,能够转化土壤中多种形态的难溶无机磷为可溶性的CaHPO4,显著增加土壤中CaHPO4的含量。同时阻滞了Ca8H2(PO4)6·5H2O、AlPO4和和FePO4等难溶无机磷的固定。

Isolation and identification of a novel phosphate-dissolving strain Penicillium aculeatum Z32 and its colonization and phosphate-dissolving characteristics in soil

Objective]To obtain new strains that can dissolving phosphate,we screened and identified a new strain Z32 capabile of phosphate dissolving from soil samples.We studied the colonization and phosphate-dissolving characteristics of Z32 in soil.Methods]Morphological,growth on various media,and ITS rDNA sequences homology analysis were performed to identify the strain Z32.We conducted a soil experiment in pot to investigate the colonization and the capbility of the different forms of inorganic phosphorus trans...