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Genetic variation in rural and urban populations of Epipactis helleborine (L.) Crantz. (Orchidaceae) in Britain 总被引:1,自引:0,他引:1
P.M. HOLLINGSWORTH J.H. DICKSON 《Botanical journal of the Linnean Society. Linnean Society of London》1997,123(4):321-331
Genetic variation in Epipactis helleborine in the British Isles was assessed using starch gel electrophoresis of isozymes; 273 individuals were sampled from 13 populations and examined for genetic variation using eight enzyme systems encoded for by 13 loci. Overall, 46% of the loci examined were polymorphic, with an average of 1.69 alleles per locus. Within populations, a mean of 33% of the loci were polymorphic, with a mean number of 1.46 alleles per locus. Levels of genetic variation were compared between urban and well established rural populations to assess the genetic consequences of colonization of the urban sites. The average levels of genetic variation detected in urban populations were lower than that found in rural populations, although there was a much greater range of variation among the urban populations. Large urban populations actually have patterns of variation similar to rural populations and show evidence of multiple founders. This indicates that the high dispersibility of Epipactis seeds can in some cases overcome the predicted loss of genetic variation associated with founder effects during colonization. Small urban populations, however, show significantly lower levels of genetic variation compared with these large urban populations and the rural populations, and it seems likely that this is attributable to single founding events and/or genetic drift. 相似文献
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David O. Simelane 《Biocontrol Science and Technology》2006,16(9):971-979
Compatibility between the root-feeding agent Longitarsus bethae Savini & Escalona and the varieties of the target weed Lantana camara L. could influence the chances of successful establishment and effectiveness of this agent as a biocontrol agent of lantana in South Africa. Laboratory experiments were conducted to determine the influence of major South African lantana varieties on feeding, colonization, oviposition preference and larval survival of L. bethae. Feeding preference and the number of adults per plant varied among the varieties. The most damaged and colonized varieties were 150 Orange, 009 Light Pink and 113 Dark Pink. Variety 018 Dark Pink was the least damaged, while 021 Total Pink and 029 White Pink were the least colonized. Females laid the highest number of eggs on variety 150 Orange. The lowest number of eggs was recorded on Australian Richmond Pink and the closely related species Lippia wilmsii. Larval survival differed significantly among the varieties. The highest survival was recorded on 150 Orange. Duration of development also varied significantly among the varieties, with the slowest rate of development recorded on variety 029 Light Pink and the quickest on variety 015 White Yellow. Although variations in female body size were less significant among varieties, males emerging from variety 150 Orange were slightly smaller than those emerged from other varieties. There was a significant tendency for females to select varieties that enhanced the survival of their offspring, which met the predictions of the preference-performance hypothesis. The data suggest that the chances of establishment and successful control of the weed are expected to be much greater on those varieties that were preferred for oviposition and provided better larval performance. 相似文献
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A rapid altitudinal range expansion in the pine processionary moth produced by the 2003 climatic anomaly 总被引:2,自引:0,他引:2
ANDREA BATTISTI MICHAEL STASTNY† EMILIANO BUFFO STIG LARSSON‡ 《Global Change Biology》2006,12(4):662-671
Climatic anomalies may produce, or accelerate, geographic range expansions of species limited by temperature or other climatic variables. Most such expansions are only temporary, before the prevailing climatic conditions drive the founder populations extinct. In contrast, here, we report a recent rapid shift of the range limit during the record hot summer of 2003 in southern Europe that has the potential to be both permanent, and to have important implications on species range dynamics in general. The winter pine processionary moth (Thaumetopoea pityocampa), an important pine defoliator whose larvae feed in colonies during the winter, is limited in its distribution by winter temperatures. In the last three decades, warmer winters have led to a gradual but substantial expansion of its range both latitudinally and altitudinally. In the summer of 2003, T. pityocampa underwent an extraordinary expansion to high elevation pine stands in the Italian Alps; its altitudinal range limit increased by one third of the total altitudinal expansion over the previous three decades. In an experiment, we found flight activity of newly emerged females to increase with temperature. By determining a threshold temperature for flight take‐offs under controlled conditions, we calculated that the nights above the threshold temperature were over five times more frequent, and considerably warmer, at the range limit in 2003 than in an average year. We therefore attribute the colonization of extreme, high‐elevation sites to increased nocturnal dispersal of females during the unusually warm night temperatures in June – August 2003. Importantly, the colonies established at extreme sites survived the winter and produced offspring in 2004, although the range did not expand further because of low night temperatures that year. We discuss several life‐history characteristics of T. pityocampa that maximize the likelihood of population persistence at the new range limit. As global warming continues and climatic anomalies are predicted to become more frequent, our results draw attention to the importance of extreme climatic events in the range formation of phytophagous insects. 相似文献
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The seven currently recognized species of Geranium endemic to the Hawaiian Islands are unusual in their shrubby or arborescent habit and unlobed, parallel-veined leaves rather than the palmately cleft or lobed leaves and herbaceous habit typical of the genus. Their placement within the genus and their biogeographic source have been obscured by this morphological distictiveness and the limited resolution of relationships on the basis of morphology in the very speciose subgenus Geranium. Phylogenetic analysis of rbcL gene sequences provides strong support for the monophyly of the Hawaiian group, and indicates that the Hawaiian clade is deeply nested within section Geranium rather than comprising a separate section. The continental relatives studied to date with the greatest similarity in sequence to the Hawaiian group are native to the Americas rather than Asia or the Pacific. The Hawaiian species are extremely similar to one another in rbcL sequence, while the tree topology obtained is consistent with a basal position for Geranium arboreum within the group. 相似文献
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U. Schotte T. Hoffmann N.G. Schwarz S. Rojak J. Lusingu D. Minja J. Kaseka J. Mbwana S. Gesase J. May D. Dekker H. Frickmann 《Letters in applied microbiology》2021,72(6):774-782
The study was performed to compare real-time PCR after nucleic acid extraction directly from stool samples as well as from samples stored and transported on Whatman papers or flocked swabs at ambient temperature in the tropics. In addition, the possible suitability for a clear determination of likely aetiological relevance of PCR-based pathogen detections based on cycle threshold (Ct) values was assessed. From 632 Tanzanian children <5 years of age with and without gastrointestinal symptoms, 466 samples were subjected to nucleic acid extraction and real-time PCR for gastrointestinal viral, bacterial and protozoan pathogens. Equal or even higher frequencies of pathogen detections from Whatman papers or flocked swabs were achieved compared with nucleic acid extraction directly from stool samples. Comparison of the Ct values showed no significant difference according to the nucleic acid extraction strategy. Also, the Ct values did not allow a decision whether a detected pathogen was associated with gastrointestinal symptoms. 相似文献
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