| 蜂毒溶血肽前体蛋白cDNA的克隆及其融合蛋白的表达 |
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| 引用本文： | 王关林,李大力,方宏筠.蜂毒溶血肽前体蛋白cDNA的克隆及其融合蛋白的表达[J].微生物学报,2001,41(2):181-185. |
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| 作者姓名： | 王关林 李大力 方宏筠 |
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| 作者单位： | 1. 辽宁师范大学生物工程研究所,大连,116029
2. 大连理工大学化工学院,大连,116012 |
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| 摘 要： | 从蜜蜂毒腺中提取总RNA ,通过RT PCR扩增得到了蜂毒溶血肽前体蛋白的cDNA ,将扩增产物克隆到 pT7Blu T载体上 ,再进一步将插入片段酶切并连接到 pUC1 1 8载体上 ,构建了重组质粒pUMP。DNA序列分析结果表明 ,克隆得到的cDNA序列与所发表序列完全相同 ,且与 β 半乳糖苷酶部分序列构成正确的读码框。含重组质粒 pUMP的大肠杆菌DH5α表达了与β 半乳糖苷酶部分序列融合的蜂毒溶血肽前体蛋白
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| 关 键 词： | 蜂毒溶血肽前体蛋白 cDNA 融合蛋白表达 |
| 文章编号： | 0001-6209(2001)02-0181-05 |
| 修稿时间： | 2000年3月6日 |
CLONING OF PROMELITTIN cDNA AND ITS EXPRESSION IN ESCHERICHIA COLI |
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| Wang Guanlin,\ Li Dali,\ Fang Hongjun.CLONING OF PROMELITTIN cDNA AND ITS EXPRESSION IN ESCHERICHIA COLI[J].Acta Microbiologica Sinica,2001,41(2):181-185. |
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| Authors: | Wang Guanlin \ Li Dali \ Fang Hongjun |
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| Institution: | Bioengineering Institute of Liaoning Normal University, Dalian 116029, China. |
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| Abstract: | The cDNA encoding promelittin was obtained from the total RNA of bee poison gland by RT-PCR and was cloned to pT7Blu-T vector. The expression vector of promelittin fused with partial sequence of beta-galactosidase was constructed by ligating the fragment inserted to pUC118. Moreover, it was expressed in the strain DH5 alpha of Escherichia coli. The result of DNA sequence analysis demonstrated that the obtained cDNA sequence was same with the published one and the reading frame of fusion gene was correct. |
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| Keywords: | Promelittin cDNA Expression fused |
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