鱼腥草抑菌有效成分的醇提、鉴定及抑菌效果研究

以大肠埃希菌抑菌效果为指标,采用正交实验的方法对鱼腥草抑菌有效成分的醇提条件进行优化,同时对鱼腥草醇提液进行分极分离,采用HPLC和GC-MS的方法对抑菌有效部的成分进行鉴定,并对该有效部的抑菌效果进行初步研究。实验结果表明鱼腥草抑菌有效成分醇提的最佳条件为醇浓度70%,温度80℃、料液比1?15,提取时间4 h,该条件下所获得的醇提液分极分离所获得的氯仿部抑菌效果最好,GC-MS和HPLC分析表明氯仿部中含有13种酚类物质及少量的鱼腥草素和甲基正壬酮,该有效部对大肠埃希菌(MIC:16.0 mg/mL)、金黄色葡萄球菌(MIC:8.0 mg/mL)、白色念珠菌(MIC:4.0 mg/mL)、黄曲霉(MIC:8.0 mg/mL)以及黑曲霉(MIC:8.0mg/mL)均有较好的抑菌效果。

The alcohol extraction,identification and effect of the antibacterial components of Houttuynia cordata Thunb

The conditions of the alcohol extraction from Houttuynia Cordata Thunb was optimized through orthogonal experiment indexed by the bacteriostatic effect.And the products were purified by liquid-liquid extraction and its components were analyzed by GC-MS and HPLC.At the same time,its antibacterial effect was tested.The result showed that the optimized conditions were 70 % alcohol,80 ℃,4 h,and feed radio 1︰15.Only the liquid purified by chloroform was effective.The GC-MS and HPLC analysis showed that the liquid extracted by chloroform contained 13 phenolic compounds and a little of sodium houttuyfonate and 2-Undecanone.The microbial resistance test proved that the antibacterial components were highly effective against Escherichia coli（MIC：16.0 mg/mL）,Staphylococcus aureus（MIC：8.0 mg/mL）,Candida albicans（MIC：4.0 mg/mL）,Aspergillus flavus（MIC：8.0 mg/mL） and Aspergillus niger（MIC：8.0 mg/mL）.