金荞麦抗菌活性研究

目的 探讨金荞麦乙醇提取物乙酸乙酯的萃取部分（待测样品）的抑菌作用,并对此部分进行分离纯化以研究其抗菌活性的物质基础。方法 与复方板蓝根颗粒做对比,采用平皿稀释法及动物实验对待测样品进行体外及体内实验,测定其对各试验菌的最小抑菌浓度（MIC）和对肺炎链球菌感染小鼠的体内保护作用.并采用多种色谱方法对此部分进行分离纯化。结果 体外抑菌试验表明,待测样品对乙型溶血性链球菌、肺炎球菌有明显的抑制作用;体内抑菌实验表明此部分对肺炎球菌菌株所致的小鼠感染有保护作用;从该活性部分分离得到8个化合物为：反式对羟基桂皮酸甲酯（trans-p-hydroxy cinnamic methyl ester,Ⅰ）,3,4-二羟基苯甲酰胺（3,4-dihydroxy benzamide,Ⅱ）,原儿茶酸（protocatechuic acid,Ⅲ）,原儿茶酸甲酯（protocatechuic acid methyl ester,Ⅳ）,木犀草素（luteolin,V）,槲皮素（quercitrin,Ⅵ）,芸香苷（rutm,Ⅶ）,（一）-表儿茶素（一）-epicatechin,Ⅷ]。结论 待测样品在体内及体外均有较强的抑菌活性，而具有抗菌作用的物质基础为酚酸类及黄酮类化合物。

Study on bacteriostasis of Fagopyrum cymosum Meisn

Objective To study on bacteriostasis of extract of Fagopyrum cymosum(Trev.) Meisn.with ethanol and ethyl acetate.Then separated this partion.Methods Compared with Banlangen,minimal inhibitory concentration(MIC) in vitro and the protection in vivo to the mice which infected with Streptococcus pneumoniae of the extract effecting on bacteria was assayed by petri dish dilution and zoopery.By solvent extraction,various method of isolation.Results This extract had different bacteriostastic activity on different Bacillus.The second of Hemolytic streptococcus and Pneumococcus were obvisously resrained.And 8 compounds were isolated and purified from this partion.Conclusions The extract of Fagopyrum cymosum(Trev.) Meisn.with ethanol and ethyl(ace)tate had obvisously resrained on the second of Hemolytic streptococcus and Pneumococcus.The compounds isolated from the partion presume bacteriostastic activity .