高脂血症患者肠道优势菌群与血清脂质水平相关性研究

目的探讨高脂血症患者肠道优势菌群变化及其与血清脂质水平的相关性。方法高脂血症患者及健康受试者各50例，采集其空腹血清样本和粪便样本，血清样本用于检测血清中总胆固醇（TC）、甘油-二酯（TG）和低密度脂蛋白胆固醇（LDL—C）的含量。应用实时定量PCR技术检测肠道内优势菌群的含量，并将其与血清脂质水平进行相关性分析。结果高脂血症患者肠道内总细菌量及拟杆菌属细菌较健康受试者组差异无统计学意义（P〉0．05），而双歧杆菌属细菌、乳杆菌属细菌及粪杆菌属细菌较健康受试者明显降低（P〈0．05），肠杆菌科细菌和肠球菌属细菌较健康受试者明显升高（P〈0．05）。高脂血症患者血清Tc与双歧杆菌属细菌、乳杆菌属细菌和粪杆菌属细菌呈现显著负相关，而与肠杆菌科细菌和肠球菌属细菌呈现显著正相关；血清LDL—C与双歧杆菌属细菌和粪杆菌属细菌呈现显著负相关，而与肠球菌属细菌呈现显著正相关；血清TG与双歧杆菌属细菌和乳杆菌属细菌呈现显著负相关，而与肠杆菌科细菌和肠球菌属细菌呈现显著正相关。结论高脂血症患者肠道优势菌群发生了明显的变化，血清脂质水平与肠道优势菌群变化具有显著相关性，提示肠道优势菌群结构的调整可改善患者血清脂质水平。

Correlations between gut predominant bacteria and serum lipids in patients with hyperlipidemia

Objective To explore the changes of gut abundant bacteria in patients with hyperlipidemia and its associations with the concentration of serum lipids. Methods 50 patients with hyperlipidemia and 50 healthy control subjects were enrolled in our present study, When the participants underwent routine physical examination, blood and feces were collected. Serum was isolated from blood and was used for the detection of total cholesterol （ TC）, triglycerides （TG） and low-density lipoprotein cholesterol （LDL-C）. The total genomic bacterial DNA was extrac- ted from feces and was used for gut abundant bacteria detection using real-time quantitative PCR. Then we analyzed the correlations between gut abundant bacteria and serum lipid in patients with hyperlipidemia. Results The a- mount of the total bacteria and Bacteroides group were not significant different between patients with hyperlipidemia and healthy control subjects （ P 〉 0.05 ）. However, Bifidobacterium spp. , Fecalibacterium prausnitzii and Lactoba- cillus spp. were present in higher copy numbers in healthy control subjects, while Enterobacteriaceae and Enterococ- cus spp. were more common in patients with hyperlipidemia （ P 〈 0.05 ）. Serum TC was correlated with Bifidobac- terium spp. , Fecalibacterium prausnitzii and Lactobacillus spp. negatively, while it was correlated with Enterobacte- riaceae and Enterococcus spp. positively. Serum LDL-C was correlated with Bifidobacterium spp. and Fecalibacteri- um prausnitzii negatively, while it was correlated with Enterococcus spp. positively. Serum TG was correlated with Bifidobacterium spp. and Lactobacillus spp. negatively, while it was correlated with Enterobacteriaceae and Entero- coccus spp. positively. Conclusion The changed gut abundant bacteria in patients with hyperlipidemia were correlated with serum lipid levels significantly, which indicated that the restoration of the gut abundant bacteria could be helpful to control serum lipid levels.