| 贫铀经口慢性染毒对小鼠肠道菌群多样性的影响 |
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| 引用本文： | 任泂,唐欢,魏泓,郝玉徽,刘晶,李蓉,粟永萍.贫铀经口慢性染毒对小鼠肠道菌群多样性的影响[J].中国微生态学杂志,2011,23(10):883-886. |
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| 作者姓名： | 任泂 唐欢 魏泓 郝玉徽 刘晶 李蓉 粟永萍 |
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| 作者单位： | 1. 第三军医大学军事预防医学院复合伤研究所 创伤烧伤与复合伤国家重点实验室,重庆,400038
2. 第三军医大学基础部实验动物学教研室,重庆,400038 |
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| 基金项目： | 全军医药卫生科研基金(08G090) |
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| 摘 要： | 目的观察贫铀经口慢性染毒对小鼠肠道菌群多样性的影响。方法通过将不同剂量的贫铀混入饲料中饲养小鼠，以小鼠肝脏和肾脏铀含量作为判断贫铀在动物体内蓄积的指标，建立贫铀经121慢性染毒小鼠模型，观察各组小鼠之间的体重变化，对各组小鼠进行基于细菌16SrRNAV6－V8区的PCR－DGGE分析。结果食入贫铀的各组小鼠肝脏和肾脏铀含量显著高于对照组（P〈0．05），各组小鼠体重差异无统计学意义（P〉0．05），各组小鼠V6－V8IXDGGE图谱的丰富度和多样性指数差异均无统计学意义（P〉0．05）。结论贫铀经日慢性染毒对小鼠肠道菌群多样性无显著影响。
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| 关 键 词： | 贫铀 慢性染毒 肠道菌群 多样性 |
The diversity of intestinal microbiota in mice after chronic oral exposure to depleted uranium |
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| REN Jiong,TANG Huan,WEI Hong,HAO Yu-hui,LIU Jing,LI Rong,SU Yong-ping.The diversity of intestinal microbiota in mice after chronic oral exposure to depleted uranium[J].Chinese Journal of Microecology,2011,23(10):883-886. |
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| Authors: | REN Jiong TANG Huan WEI Hong HAO Yu-hui LIU Jing LI Rong SU Yong-ping |
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| Institution: | REN Jiong1,TANG Huan2,WEI Hong2,HAO Yu-hui1,LIU Jing1,LI Rong1,SU Yong-ping1 (1.Institute of Combined Injury,College of Preventive Medicine,Third Military Medical University,State Key Laboratory of Trauma,Burns and Combined Injury,Chongqing 400038,China,2.Department of Laboratory Animal Science,College of Basic Medical Sciences,China) |
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| Abstract: | Objective To assess the diversity of intestinal microbiota in mice after chronic oral exposure to depleted uranium(DU). Method SPF grade KM mice were exposed to DU in food at different doses for four months to generate mice model of chronic oral exposure to DU.The uranium content in the livers and kidneys as well as the body weight in each group were tested.PCR-DGGE based on the V6-V8 region of bacterial 16S rRNA was performed.The richness and the Shannon-Wiener index were analyzed to compare the effect of ... |
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| Keywords: | Depleted uranium Chronic oral exposure Intestinal microbiota Diversity |
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