| EV71灭活疫苗（人二倍体细胞）在恒河猴婴猴模型中的免疫保护性分析 |
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| 引用本文： | 李龙,刘尚允,董承红,赵红玲,王晶晶,张莹,纳锐雄,谢忠平,崔萍芳,王丽春,廖芸,唐东红,高家红,刘龙丁,李琦涵.EV71灭活疫苗（人二倍体细胞）在恒河猴婴猴模型中的免疫保护性分析[J].中国科学:生命科学,2011,41(6):439-448. |
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| 作者姓名： | 李龙 刘尚允 董承红 赵红玲 王晶晶 张莹 纳锐雄 谢忠平 崔萍芳 王丽春 廖芸 唐东红 高家红 刘龙丁 李琦涵 |
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| 作者单位： | 中国医学科学院&北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室; |
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| 基金项目： | 国家科技重大专项课题(批准号: 2008ZX10004-014); 国家重点基础研究发展计划(批准号: 2011CB504903)资助项目 |
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| 摘 要： | 肠道病毒71型作为引起儿童群体常见传染性手足口病（HFMD）的主要病原，具有导致少量感染个体出现脑炎等神经系统病变以及相关心肺功能衰竭的病理学特性．因此其预防性疫苗的研发具有重要的公共卫生意义．在前期工作的基础上，一种EV71灭活病毒疫苗（人二倍体细胞）在本研究中基于恒河猴婴猴模型进行了相应的免疫保护性分析．以160EU剂量对2～3月龄婴猴进行0，4周免疫后，动物在第4周接受了剂量为10。～CCID50的病毒经呼吸道的攻击．对病毒攻击后动物在14天内的临床症状、血液生物学、器官病原学分布以及病理学检测的动态观察表明，经疫苗免疫的动物未出现对照动物所具有的特征性临床表现，其血液生物学及病理学检测均无异常．同时，器官病原学分布亦呈阴性．结合动物中和抗体的明确增长及对照动物的综合表现分析，本文的工作证实了该EV71灭活病毒疫苗（人二倍体细胞）在恒河猴婴猴体内的免疫保护性．
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| 关 键 词： | 肠道病毒71型 （Enterovirus 71 EV71） 灭活疫苗 免疫原性 恒河猴婴猴 |
Immunoprotection of Inactivated EV71 Vaccine Against Enterovirus in Neonatal Rhesus Monkey |
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| LI Long,LIU Shangyun,DONG Chenghong,ZHAO Hongling,WANG Jingjing,ZHANG Ying,NA Ruixiong,XIE Zhongping,CUI Pingfang,WANG Lichun,LIAO Yun,TANG Donghong,GAO Jiahong,LIU Longding & LI Qihan Institute of Medical Biology,Chinese Academy of Medical Science , Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases,Kunming ,China.Immunoprotection of Inactivated EV71 Vaccine Against Enterovirus in Neonatal Rhesus Monkey[J].Scientia Sinica Vitae,2011,41(6):439-448. |
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| Authors: | LI Long LIU Shangyun DONG Chenghong ZHAO Hongling WANG Jingjing ZHANG Ying NA Ruixiong XIE Zhongping CUI Pingfang WANG Lichun LIAO Yun TANG Donghong GAO Jiahong LIU Longding & LI Qihan Institute of Medical Biology Chinese Academy of Medical Science Peking Union Medical College Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases Kunming China |
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| Institution: | LI Long,LIU Shangyun,DONG Chenghong,ZHAO Hongling,WANG Jingjing,ZHANG Ying,NA Ruixiong,XIE Zhongping,CUI Pingfang,WANG Lichun,LIAO Yun,TANG Donghong,GAO Jiahong,LIU Longding & LI Qihan Institute of Medical Biology,Chinese Academy of Medical Science and Peking Union Medical College,Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases,Kunming 650118,China |
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| Abstract: | Present study was conducted to evaluate the immunogenicity of one formalin-inactivated EV71 vaccine in neonatal rhesus monkeys(Macaca mulatta).Groups of four monkeys were immunized with either EV71 vaccine or mock by intra-muscular(IM,using needle) routes.Following primary immunization with 160EU vaccine given IM,the monkeys were boosted after 1 month with 160EU vaccine given IM.After the booster,most of the monkeys sero-converted and developed EV71 neutralizing antibodies.Importantly however,following a sh... |
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| Keywords: | enterovirus 71 inactivated vaccine immunogenicity nounatal rhesus monkey |
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