金银花五个品系的RAPD分析及DNA指纹图谱的建立

运用RAPD技术,对5个金银花品系进行遗传多样性研究并构建这5个金银花品系的DNA指纹图谱。从80个引物中筛选出25个带纹清晰,多态性好的引物用于实验。其中,引物SBSD06的扩增条带可以清楚明确区分5个品系,建立其DNA指纹图谱。在清晰、稳定出现的170条带中,153条带具有多态性。按UPGMA法进行聚类分析,计算其遗传相似系数,结果显示,金银花5个品系聚为两类,与其形态学分类结果相符。

RAPD Analysis and Construction of DNA Fingerprints in Five Flos lonicerae Lines

RAPD technique has been used to construct DNA fingerprints and analyze genetic diversity in Flos lonicerae.From eighty primers,twenty-five primers were selected which can amplify clear and reproducible polymorphic bands.The PCR results showed that a total of 170 bands were amplified,of which 153 bands were polymorphic.Interestingly,primer SBSD06 generated distinct fingerprinting patterns that could distinguish each of the five lines.Genetic similarity correlation and the cluster analysis indicated the abundant genetic diversity in five F.lonicerae lines.A dendrogram was constructed based on genetic distances using UPGMA method.The dendogram reflected that five F.lonicerae lines clustered into two groups,and this was in accordance with the result of morphologic taxa.