莲藕组织总RNA的快速提取方法

莲藕组织富含多糖、脂质、酚类等物质，用一般的方法较难提取高质量的RNA。在改进前人方法的基础上，建立了一种高效、简单的CTAB-LiCl提取法，能快速提取高质量的莲藕组织总RNA，并且产率高、完整性好、纯度高，能进一步满足RT-PCR等分子生物学实验的需要。此外，该方法也适用于其它富含多糖、脂质、酚类等物质的植物组织总RNA的提取。

A Method of Total RNA Isolation in Lotus Tissues

The lotus tissues contain a lot of secondary metabolites such as polysacharides ,phenolic compounds,lipids etc. Therefore from which it is difficult to isolate high-quality RNA with ordinary-methods. According to the prior researches ,a highly efficient RNA extraction method for lotus tissues was modified. RNA,which is high concentration,nonconspicuous degradation and good purity, could be attained by using a novel and simple CTAB-LiCl-based extraction method. This method is also sufficient for further RT-PCR and other molecular biology experiments. Therefore, it is also useful for the RNA extraction of other plant materials which have plenty of polysaccharide, phenolic compounds.