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黄瓜果瘤的遗传及SSR 标记
引用本文:王桂玲,秦智伟,周秀艳,赵咫云.黄瓜果瘤的遗传及SSR 标记[J].植物学报,2007,24(2):168-172.
作者姓名:王桂玲  秦智伟  周秀艳  赵咫云
作者单位:1 东北农业大学园艺学院, 哈尔滨 150030; 2 哈尔滨师范大学, 哈尔滨 150080
基金项目:黑龙江省自然科学基金,黑龙江省教育厅海外学人科研(合作)基金
摘    要:通过对以荷兰温室无瘤黄瓜(Cucumis sativus )品种Z1和Z3为母本(tutu), 有瘤黄瓜品系东农129为父本(TuTu)的杂交后代F1和F2的统计分析, 结果表明: 在F1代, 2个组合果实都有果瘤, 有果瘤为显性; F2代果实有瘤与无瘤呈现分离, 其比例分别是2.92:1和2.95:1, 表明Tu基因是独立遗传的, 即有瘤(Tu)对无瘤(tu)为显性。为了获得与黄瓜果瘤基因连锁的SSR(simple sequence repeat)标记, 从129×Z3 F2群体中选取有瘤、无瘤单株的DNA各10个, 构建有瘤、无瘤近等基因池。用86对SSR引物在亲本及DNA混合池之间进行筛选, 并对F2群体的75个单株进行验证。筛选得到了5对与果瘤相关的SSR引物, 经MAPMAKER/EXP version 3.0b软件分析, 其中CSWGATT01B、CSWGATT01C、CSCT335和CSWGATT01A四个标记位于同一连锁群上, Tu基因位于这4个标记构建的连锁群中, 具体位置为CSWGATT01C-Tu-CSCT335, 距离两侧标记的遗传距离分别为20.0 cM和14.1 cM。

关 键 词:黄瓜  遗传  分子标记  SSR  果瘤
收稿时间:2006-06-21
修稿时间:2006-08-16

Genetic Analysis and SSR Markers of Tuberculate Trait in Cucumis sativus
Guiling Wang,Zhiwei Qin,Xiuyan Zhou,Zhiyun Zhao.Genetic Analysis and SSR Markers of Tuberculate Trait in Cucumis sativus[J].Bulletin of Botany,2007,24(2):168-172.
Authors:Guiling Wang  Zhiwei Qin  Xiuyan Zhou  Zhiyun Zhao
Institution:1Northeast Agricultural University, Harbin 150030, China; 2Harbin Normal University, Harbin 150080, China
Abstract:To analyze the heredity of tuberculate trait in Cucumis sativus, we constructed 2 populations. Female parents were 2 varieties without tuberculate, Z1 and Z3, derived from Dutch species, and planted in a greenhouse. The male parent was Dongnong 129, a variety with tuberculate. The fruit of the F1 family from the 2 crosses had tuberculate, which indicated that the tuberculate trait was dominant. The tuberculate trait and no tuberculate trait segregated in the F2 family. The segregation ratios were 2.92 : 1 and 2.95 : 1 for the 2 families, respectively, which revealed that the gene Tu played a role in independent inheritance. A total of 86 pairs of simple sequence repeat primers were screened in 2 near-isogenic pools created by mixing DNA of 10 individuals randomly chosen from the F2 family according to tuberculate or no tuberculate trait. Five separate amplification products obtained by use of 5 pairs of the primers and 4 products — CSWGATT01B, CSWGATT01C, CSCT335, and CSWGATT01A — were mapped to the same linkage group by use of MAPMAKER/EXP, version 3.0b. Tu in the linkage group located between CSWGATT01C and CSCT335 at 20.0 cM and 14.1 cM from the markers, respectively.
Keywords:,
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