| S-扁桃酸脱氢酶基因的克隆及表达 |
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| 引用本文： | 曾贞,杨军方,杨成丽,王鹏,李大力.S-扁桃酸脱氢酶基因的克隆及表达[J].中国生物工程杂志,2012,32(2):29-32. |
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| 作者姓名： | 曾贞 杨军方 杨成丽 王鹏 李大力 |
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| 作者单位： | 南京理工大学生物工程系 南京 210094 |
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| 摘 要： | S-扁桃酸脱氢酶能够选择性催化S-扁桃酸生成苯甲酰甲酸。通过PCR扩增获得Pseudomonas p utida NUST的S-扁桃酸脱氢酶全长基因(mdlA),并构建了表达载体pET30a(+)-mdlA,转化大肠杆菌E.coli BL21(DE3)后,经异丙基-β-D-硫代吡喃半乳糖苷(IPTG)诱导获得表达,SDS-PAGE结果显示表达蛋白为43kDa。所以工程菌细胞具有转化S-扁桃酸生成苯甲酰甲酸能力。
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| 关 键 词： | 生物催化 S-扁桃酸脱氢酶 克隆 表达 |
| 收稿时间： | 2011-10-31 |
| 修稿时间： | 2011-12-01 |
Cloning and Expression of S-mandelate Dehydrogenase Gene |
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| ZENG Zhen
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YANG Jun-fang
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YANG Cheng-li
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WANG Peng
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LI Da-li.Cloning and Expression of S-mandelate Dehydrogenase Gene[J].China Biotechnology,2012,32(2):29-32. |
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| Authors: | ZENG Zhen YANG Jun-fang YANG Cheng-li WANG Peng LI Da-li |
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| Institution: | (Department of Bioengineering,Nanjing University of Science and Technology,Nanjing 210094,China) |
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| Abstract: | S-mandelate dehydrogenase(SMDH) can catalyze S-mandelic acid to benzoylformic acid.The SMDH nucleotide gene(mdlA)was cloned from DNA of Pseudomonas putida NUST by PCR,and the amplicon was inserted into prokaryotic expression vector pET-30a(+).This recombinant was transformed into E.coli BL21(DE3) and then highly expressed by induction of IPTG.The result of SDS-PAGE showed that the molecular weight of cloned SMDH was about 43kDa.The recombinant strain could catalyze S-mandelate to benzoylformic acid. |
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| Keywords: | Biocatalyst S-Mandelate dehydrogenase Cloning Expression |
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