两种树脂对固相法合成丙型肝炎疫苗多肽的影响

本实验采用固相法合成丙型肝炎疫苗多肽，比较Wang树脂和二氯三苯甲基树脂（以下简称二氯树脂）作为载体时的连接率以及目标肽的产率和纯度。通过质谱鉴定分析所得目标多肽的分子量，用茚三酮法对两种树脂与氨基酸的连接率进行比较，用反相高效液相色谱（RP-HPLC）进行粗肽的分析和纯化。结果显示以二氯树脂作载体，第一个氨基酸的连接率，以及目标肽的纯度和产率都要明显高于Wang树脂，因此二氯树脂作载体合成丙型肝炎疫苗多肽更适合大规模工业化生产的要求。

The Influence of Two Resins on Synthesizing HCV Polypeptides by Solid-phase Method

The solid-phase method to synthesis HCV polypetides was used.The connecting rate and the polypeptides' productivity and purity were compared when Wang resin and 2-chlorotrity choloride resin acting as vectors.By the identification of mass spectrum,the products' molecular weight was analysed.By ninhydrin method we compared to the connecting rate between two resins and amino acid.By RP-HPLC we carried out the analysis and purity of hetero peptides.The result showed the first amino acid's connecting rate along with the purity and productivity which we need when 2-chlorotrityl choloride resin acted as vector was notably higher than those when Wang resin acted as vector.So 2-chlorotrityl choloride as vector is used to synthesize HCV polypeptides,which adapts to the demand of big scale's industrialization production.