| 基于MegaBACE 1000的荧光(marker)开发 |
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| 引用本文： | 刘贤德,张国范.基于MegaBACE 1000的荧光(marker)开发[J].中国生物工程杂志,2009,29(12):90-93. |
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| 作者姓名： | 刘贤德 张国范 |
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| 作者单位： | 1.中国科学院海洋研究所 青岛 266071
2.福建省高校水产科学技术与食品安全重点实验室/集美大学水产学院 厦门 361021 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，福建省青年人才项目 |
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| 摘 要： | MegaBACETM 1000 DNA测序仪是由美国Pharmacia公司生产的一种高通量的DNA测序仪，利用这套测序仪，可以进行DNA测序、遗传分析等相关研究。但该公司生产的用于遗传分析的DNA标记物（marker）（ET550-R Size Standards）价格不菲，出于节省成本的考虑，自行开发了荧光标记物，经过验证，这个标记完全可以在MegaBACE 1000 DNA测序仪上使用。利用这套标记物和自己开发的标记物识别软件，构建了一套基于MegaBACE 1000 DNA测序仪的改良的、高通量的AFLP操作流程。
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| 关 键 词： | MegaBACE marker AFLP |
| 收稿时间： | 2009-05-07 |
| 修稿时间： | 2009-07-24 |
The Fluorescent Marker Development Based on MegaBACE 1000 DNA Sequencing System |
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| LIU Xian-de,ZHANG Guo-fan.The Fluorescent Marker Development Based on MegaBACE 1000 DNA Sequencing System[J].China Biotechnology,2009,29(12):90-93. |
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| Authors: | LIU Xian-de ZHANG Guo-fan |
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| Institution: | 1.Chinese Academy of Sciences, Institute of Oceanology, Qingdao 266071, China
2.Key Laboratory of Science and Technology for Aquaculture and Food Safety of Fujian Province University,Fisheries College, Jimei University, Xiamen 361021, China |
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| Abstract: | The MegaBACE~(TM) 1000 DNA Sequencing System was made by Amersham Pharmacia, which is a high throughput fluorescence-based DNA sequencer that can be used for DNA sequencing and genetic analysis etc. But the markers (ET550-R Size Standards) produced by this corporation which are used for genetic analysis in this instrument are very expensive. In order to cut the cost, a set of markers were developed and after authentication, that can be used in the MegaBACE 1000 DNA sequencer. Using these markers and our own recognition software, an improved, high throughput AFLP work protocol based on MegaBACE~(TM) 1000 were constructed. |
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| Keywords: | MegaBACE AFLP |
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