Serological detection of Sharka quarantine disease (Plum pox virus) on stone fruit trees in Golestan province |
| |
| Authors: | Hasan Hosseinzadeh Saeid Nasrollanejad Zahra Dordiani Vahid Jamshidnezhad |
| |
| Institution: | 1. Department of Plant Protection , Gorgan University of Agricultural Sciences and Natural Resources , Gorgan , Iran hasan_dazil2004@yahoo.com;3. Department of Plant Protection , Gorgan University of Agricultural Sciences and Natural Resources , Gorgan , Iran;4. Department of Plant Protection , University College of Abureihan, University of Tehran , Tehran , Iran |
| |
| Abstract: | Sharka disease is one of the most damaging diseases of fruit trees in the world, which is caused by Plum pox virus (PPV) that belongs to the genus Potyvirus in the family Potyviridae. Each year, this virus decreases the yield and causes substantial economic damages to its host plants worldwide. This virus is quarantined in Iran but in recent years, suspicious symptoms of the disease were observed in different grown areas, such as Golestan province. During 2010, 420 samples with mosaic, chlorosis, necrosis, ring pattern, blotches, etc. symptoms were collected from the gardens in Golestan province that included 100 samples from plum, 100 from peach and 240 from nectarine. These samples were evaluated using a double-antibody sandwich-ELISA (DAS-ELISA) method and a polyclonal antibody. The results of this survey indicated that among the total of 420 samples, none of them showed positive reaction in DAS-ELISA test. |
| |
| Keywords: | Sharka PPV stone fruits Golestan province DAS-ELISA |
|
|
