Controlled Cervical Laceration Injury in Mice |
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| Authors: | Yi Ping Zhang Melissa J Walker Lisa B E Shields Xiaofei Wang Chandler L Walker Xiao-Ming Xu Christopher B Shields |
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| Institution: | 1.Norton Neuroscience Institute, Norton Healthcare;2.Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Medical Neuroscience Graduate Program, and Department of Anatomy and Cell Biology, Indiana University School of Medicine |
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| Abstract: | Use of genetically modified mice enhances our understanding of molecular mechanisms underlying several neurological disorders such as a spinal cord injury (SCI). Freehand manual control used to produce a laceration model of SCI creates inconsistent injuries often associated with a crush or contusion component and, therefore, a novel technique was developed. Our model of cervical laceration SCI has resolved inherent difficulties with the freehand method by incorporating 1) cervical vertebral stabilization by vertebral facet fixation, 2) enhanced spinal cord exposure, and 3) creation of a reproducible laceration of the spinal cord using an oscillating blade with an accuracy of ±0.01 mm in depth without associated contusion. Compared to the standard methods of creating a SCI laceration such as freehand use of a scalpel or scissors, our method has produced a consistent lesion. This method is useful for studies on axonal regeneration of corticospinal, rubrospinal, and dorsal ascending tracts. |
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| Keywords: | Medicine Issue 75 Neurobiology Anatomy Physiology Neuroscience Immunology Infection Surgery Nervous System Diseases Diagnosis Therapeutics Surgical Procedures Operative Investigative Techniques spine spinal cord injury SCI mouse laceration stabilization axonal regeneration injury mice animal model surgical techniques |
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