| 中缅树鼩微卫星分子标记的筛选 |
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| 引用本文： | 李婧潇,王新兴,王文广,孙晓梅,代解杰.中缅树鼩微卫星分子标记的筛选[J].中国实验动物学报,2011,19(4):312-315. |
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| 作者姓名： | 李婧潇 王新兴 王文广 孙晓梅 代解杰 |
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| 作者单位： | 中国医学科学院北京协和医学院医学生物学研究所,昆明,650118 |
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| 基金项目： | 国家科技支撑计划项目(编号:2009BAI83B02); 云南省科技基础条件平台建设计划项目(编号:2006PT07-2) |
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| 摘 要： | 目的 筛选中缅树鼩微卫星分子标记,逐步填补中缅树鼩特异性遗传标记的空白.方法 建立中缅树鼩基因小片段插入文库,利用5’端地高辛标记的(CA)15探针从约1500个菌落中选出36个阳性克隆.对这些克隆进行测序,发现其中15个含有重复序列,其中1个为重复克隆,1个因两端序列太短而不能设计引物.结果 用Primer3软件设计...
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| 关 键 词： | 树鼩 微卫星 分子标记 |
Isolation of microsatellite markers in tree shrews(Tupaia belangeri chinensis) |
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| LI Jing-xiao,WANG Xin-xing,WANG Wen-guang,SUN Xiao-mei,DAI Jie-jie.Isolation of microsatellite markers in tree shrews(Tupaia belangeri chinensis)[J].Acta Laboratorium Animalis Scientia Sinica,2011,19(4):312-315. |
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| Authors: | LI Jing-xiao WANG Xin-xing WANG Wen-guang SUN Xiao-mei DAI Jie-jie |
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| Institution: | LI Jing-xiao,WANG Xin-xing,WANG Wen-guang,SUN Xiao-mei,DAI Jie-jie(Institute of Medical Biology,Chinese Academy of Medical Science and Peking Union Medical College,Kunming 650118,China) |
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| Abstract: | Objective To isolate microsatellite markers in tree shrews(Tupaia belangeri chinensis),and to fill the gap of lack of specific genetic markers of tree shrews.Methods A partial genomic library was constructed in tree shrews.Thirty-six positive clones were isolated from screening about 1500 clones of the genomic library with(CA)15 probe labelled at the 5'end with digoxin.Sequences of these clones and 15 microsatellites were isolated which included one repeated clones and one short flank sequence.Results The o... |
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| Keywords: | Tree shrew Microsatellite Molecular marker |
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