| 甘草水溶物诱导MGC-803细胞凋亡中胞内Ca~(2+)、pH与线粒体△ψ_m的变化 |
| |
| 引用本文： | 马靖,李玉梅,庞大本,徐安龙.甘草水溶物诱导MGC-803细胞凋亡中胞内Ca~(2+)、pH与线粒体△ψ_m的变化[J].中国生物化学与分子生物学报,2000,16(4):556-558. |
| |
| 作者姓名： | 马靖 李玉梅 庞大本 徐安龙 |
| |
| 作者单位： | 马靖（中山大学生命科学学院生物化学系广州
510275）;李玉梅（中国中医研究院基础理论研究所北京 100700）;庞大本（中国中医研究院基础理论研究所北京 100700）;徐安龙（中山大学生命科学学院生物化学系广州 510275） |
| |
| 基金项目： | 广州市科委重点项目(97-Z-12-01)和国家新药博士基金(96-901-06-36)资助 |
| |
| 摘 要： | Changes of intracellular Ca 2+ ,pH value and mitochondria membrane potential(△Ψ m) in the apoptosis of MGC 803 cells induced by water soluble constituents of Glycyrrhiza uralensis Fisch(WSCG) were investigated.MGC 803 cells were incubated with 0.5,0.75,1.0 and 1.5 g/L WSCG for 1,4,7,11,15,19 and 23 hours respectively.The percentage of apoptosis and intracellular Ca 2+ content increased in a dose and time dependent manner,except that the intracellular Ca 2+ content of 1.5 g/L WSCG treated cells started to decrease after 11 hours.The cells were alkalized after various treatments,except that 1.5 g/L WSCG treated cells turned to acidify after 11 hours.It was found that the mitochondria △Ψ m of all treated cells decreased drastically at the first hour,then kept decreasing slowly until the 23rd hour.The results indicated that intracellular Ca 2+ ,pH and mitochondria △Ψ m all played pivotal roles in the apoptosis of MGC 803 cells induced by WSCG.
|
| 收稿时间： | 2000-08-20 |
| 修稿时间： | 1999年9月24日 |
Changes of Intracellular Ca2+ pH Value and Mitochdria in the
Apoptosis of MGC-803 Cells Induced by Glycyrrhiza uralensis Fish |
| |
| MA Jing,LI Yu mei ,PAN Da ben ,XU An long.Changes of Intracellular Ca2+ pH Value and Mitochdria in the
Apoptosis of MGC-803 Cells Induced by Glycyrrhiza uralensis Fish[J].Chinese Journal of Biochemistry and Molecular Biology,2000,16(4):556-558. |
| |
| Authors: | MA Jing LI Yu mei PAN Da ben XU An long |
| |
| Institution: | (Department of Biochemistry,College of Life Sciences,Zhongshan University,Guangzhou 510275,China; 1) Institute of Traditional Chinese Medicine,Beijing 100700,China |
| |
| Abstract: | Changes of intracellular Ca 2+ ,pH value and mitochondria membrane potential(△Ψ m) in the apoptosis of MGC 803 cells induced by water soluble constituents of Glycyrrhiza uralensis Fisch(WSCG) were investigated.MGC 803 cells were incubated with 0.5,0.75,1.0 and 1.5 g/L WSCG for 1,4,7,11,15,19 and 23 hours respectively.The percentage of apoptosis and intracellular Ca 2+ content increased in a dose and time dependent manner,except that the intracellular Ca 2+ content of 1.5 g/L WSCG treated cells started to decrease after 11 hours.The cells were alkalized after various treatments,except that 1.5 g/L WSCG treated cells turned to acidify after 11 hours.It was found that the mitochondria △Ψ m of all treated cells decreased drastically at the first hour,then kept decreasing slowly until the 23rd hour.The results indicated that intracellular Ca 2+ ,pH and mitochondria △Ψ m all played pivotal roles in the apoptosis of MGC 803 cells induced by WSCG. |
| |
| Keywords: | |
|
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
|
