| 亲水化法稳定超氧化物歧化酶 |
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| 引用本文： | 梁涌涛,刘卫华,孙启安,罗贵民,杨同书.亲水化法稳定超氧化物歧化酶[J].中国生物化学与分子生物学报,1994,10(5):596-599. |
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| 作者姓名： | 梁涌涛 刘卫华 孙启安 罗贵民 杨同书 |
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| 作者单位： | 吉林大学酶工程国家重点实验室 |
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| 摘 要： | 在氰基硼氢化钠存在下用乙醛酸修饰含铁超氧化物歧化酶（Ｆｅ－ＳＯＤ）的氨基可使酶的热稳定性显著提高，酶在８０℃时的半寿期增加一倍。
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| 关 键 词： | 超氧化物歧化酶 稳定化 乙醛酸 亲水化 化学修饰 |
| 收稿时间： | 1994-10-20 |
Stabilization of Iron-containing Superoxide Dismutase via Hydrophilization |
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| Liang,Yong-tao,Liu,Wei-hua,Sun,Qi-an,Luo,Gui-min,Yang,Tong-shu.Stabilization of Iron-containing Superoxide Dismutase via Hydrophilization[J].Chinese Journal of Biochemistry and Molecular Biology,1994,10(5):596-599. |
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| Authors: | Liang Yong-tao Liu Wei-hua Sun Qi-an Luo Gui-min Yang Tong-shu |
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| Institution: | (The National Laboratory of Enzyme Engineering, Jilin University, Changchun 130023 |
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| Abstract: | Reductive alkylation of amino groups in iron-containing superoxide dismutase with glyoxylic acid in the presence of sodium cyanoborohydride results in great stabilization of the enzyme against irreversible thermoinactivation. At 80℃ the half life of modified enzyme is about 2times longer than that of native one. |
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| Keywords: | Fe-SOD Stabilization Glyoxylic acid Reductive alkylation Hydrophilization Chemical modification |
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