基于HT-SELEX筛选的节球藻毒素-R适配体的鉴定

节球藻毒素-R（nodularin-R，NOD-R）是具有强烈肝毒性的蓝藻毒素。然而，现有的NOD-R检测技术均存在一定的局限性，亟待开发一种新的检测方法。本文利用指数富集的配基系统进化（systematic evolution of ligands by exponential enrichment，SELEX）技术结合高通量测序（HT-SELEX），筛选出NOD-R特异的高亲和力适配体。生物膜干涉（biolayer interferometry，BLI）技术对适配体的鉴定结果表明，适配体H62与NOD-R之间的亲和力最高，达到了168 nM。而且，该适配体不与其他毒素结合，具有较高的特异性。以上结果表明，适配体H62有望作为NOD-R检测新方法——适配体生物传感器的分子识别元件。

The Characterization of Nodularin-R Specific Aptamer Obtained from HT-SELEX

Nodularin-R (NOD-R), a cyanophycean toxin, is a potent hepatotoxin. However, there are different limitations of the detection methods for NOD-R. As a result, there is still an urgent need to develop a novel approach for the detection of NOD-R. Here, we combined High Throughput Sequencing (HTS) with Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method (HT-SELEX), and selected aptamer that bound with high affinity and specificity to NOD-R. Biolayer interferometry (BLI) assays showed that aptamer H62 was the best one targeting NOD-R. The binding affinity between aptamer H62 and NOD-R was as high as 168 nM. Furthermore, aptamer H62 bound with high specificity to NOD-R. These results indicate that aptamer H62 would be a new molecular recognition element and provide clues for the construction of aptasensor specializing in NOD-R detection.