| 富硒大蒜含硒蛋白的分离、鉴定和生物活性研究 |
| |
| 引用本文： | 王卫真,唐家骏,彭安.富硒大蒜含硒蛋白的分离、鉴定和生物活性研究[J].中国生物化学与分子生物学报,1989,5(3):229-234. |
| |
| 作者姓名： | 王卫真 唐家骏 彭安 |
| |
| 作者单位： | 中国科学院生态环境研究中心，中国科学院生态环境研究中心，中国科学院生态环境研究中心 北京 广西南宁市，广西肿瘤研究所生化室，北京，北京 |
| |
| 基金项目： | 中国科学院青年奖励基金项目，中科院科发基字235基金 |
| |
| 摘 要： | 本文报道了富硒大蒜含硒蛋白的分离、鉴定和生物活性研究。富硒大蒜水溶性蛋白多为酸性蛋白。通过Sephadex G—200柱层析和聚丙烯酰胺梯度凝胶电泳分离出两种分子量约为30,000和33,000的酸性含硒蛋白。大蒜含硒蛋白和亚硒酸钠一样,具有提高含硒谷胱甘肽过氧化物酶(SeGSHpx)活性,促进软骨细胞生长的生理效应。实验结果提示,富硒大蒜有可能成为良好的补硒来源。
|
| 关 键 词： | 大蒜 含硒蛋白 谷胱甘肽过氧化物酶 软骨细胞 |
| 收稿时间： | 1989-06-20 |
The Isolation, Identification and the Bioactivities of Se-Proteins in the Selenium Rich Garlic |
| |
| Wang,Wei-zhen Tang,Jia-jun Peng,An.The Isolation, Identification and the Bioactivities of Se-Proteins in the Selenium Rich Garlic[J].Chinese Journal of Biochemistry and Molecular Biology,1989,5(3):229-234. |
| |
| Authors: | Wang Wei-zhen Tang Jia-jun Peng An |
| |
| Institution: | (The Research Center for Eco-Environmental Sciences, Academia Sinica, Beijing |
| |
| Abstract: | Two acidic Se-proteins with molecular weights of 30,000 and 33, 000 were isolated by Sephadex G-200 chromatography and gradient gel electropho-resis. Se-cystine (or Se-cysteine) and Se-methionine were found in both of these two proteins. Most of the garlic Se-proteins were acidic.Supplementation of the garlic Se-proteins (54.0 ppm Se, dry weight) extract in drinking water to mice for one week increased the bioactivity of glutathione pero-xidase and promoted growth of chondrocytes were found. The results showed that the Se rich garlic may be used as a good source for enriching the Se content in foods. |
| |
| Keywords: | Garlic Selenium-protein Clutathiode peroxidase Chondrocyte |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
