| DENV2海南分离株NS1全序列测定和生物信息学分析 |
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| 引用本文： | 周俊梅,方丹云,梁瑜,尹悦,江丽芳.DENV2海南分离株NS1全序列测定和生物信息学分析[J].生物技术,2009,19(3). |
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| 作者姓名： | 周俊梅 方丹云 梁瑜 尹悦 江丽芳 |
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| 作者单位： | 中山大学中山医学院微生物学教研室,广东,广州,510080 |
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| 基金项目： | 广东省自然科学基金团队项目，广东省自然科学基金 |
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| 摘 要： | 目的:对登革病毒2型海南分离株NS1全序列进行测定和生物信息学分析,了解其系统 进化和分子流行病学特征.方法:采用RT-PCR法扩增D2-Hainan全长NS1基因,将其克隆入载 体pPIcZáB,测序,采用相关软件进行生物信息学分析.结果:获得D2-H ainan株NS1全长基 因1056bp,其序列与登革病毒2型NGC株的同源性为95%.系统进化树分析显示该毒株与登革 病毒2型China04株的亲缘关系最近.初步分析了NS1蛋白的氨基酸序列特征和二级结构.结论:登革病毒流行株存在地域性和复杂性,对D2-Hainan株NS1基因及其编 码蛋白信息特征的了解,有助于进一步研究其基因特征与病毒毒力的关系.
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| 关 键 词： | 登革病毒 NS1基因 生物信息学 |
The Gene Sequence Determination and Bioinformatic Analysis of the Nonstructure Protein 1 of Dengue 2 Virus Strain Isolated in Hainan |
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| ZHOU Jun-mei,FANG Dan-yun,LIANG Yu,YIN Yue,JIANG Li-fang.The Gene Sequence Determination and Bioinformatic Analysis of the Nonstructure Protein 1 of Dengue 2 Virus Strain Isolated in Hainan[J].Biotechnology,2009,19(3). |
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| Authors: | ZHOU Jun-mei FANG Dan-yun LIANG Yu YIN Yue JIANG Li-fang |
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| Institution: | ZHOU Jun-mei,FANG Dan-yun,LIANG Yu,YIN Yue,JIANG Li-fang(Department of Medical Microbiology,Basic Medical College,Sun Yat-sen University,Guangzhou 510080,China) |
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| Abstract: | Objective:Determine and analyze the entire sequence of the NS1 gene of dengue 2 virus strain isolated in Hainan which is to understand the characteristic of its molecular evolution.Method:The nucleotides of NS1 of D2-Hainan was amplified by RT-PCR and the sequence was determined by Sanger method.Then the gene and the encoding protein were analyzed by BLAST,MEGA,Clustal X,nnPredict,HNN,and DAS software.Result: It showed that the whole nucleotide sequence of D2-Hainan NS1 was 1 056bp and had the 95% homology ... |
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| Keywords: | Dengue virus nonstructure protein 1 bioinformatics |
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