| 液体悬浮培养促进蔓茎堇菜高效再生的研究 |
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| 引用本文： | 代容春,何文锦,林荣华,朱锦懋,陈由强.液体悬浮培养促进蔓茎堇菜高效再生的研究[J].生物技术,2003,13(4):25-26. |
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| 作者姓名： | 代容春 何文锦 林荣华 朱锦懋 陈由强 |
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| 作者单位： | 福建师范大学生物工程学院,福建,福州,350007 |
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| 基金项目： | 福建省自然科学基金资助项目(B0210008) |
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| 摘 要： | 液体悬浮培养具有促进蔓茎堇菜芽分化的作用。以叶片为外植体在培养基MS 2，4-D1.5 ZT1.0中诱导出生长良好的愈伤组织。愈伤组织在液体培养基MS 6-BA1.0 NAA0.25中振荡培养12d后（转速为90r/min），转入固体培养基MS 6-BA2.0 NAA0.5中继续培养14d，芽分化率可达97.5%，转接至生根培养基MS NAA2.0 6-BA0.25中培养1个月后可移栽，成活率在90%以上。
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| 关 键 词： | 液体悬浮培养 蔓茎堇菜 植株再生 外植体 培养基 |
| 文章编号： | 1004-311X(2003)04-0025-02 |
| 修稿时间： | 2003年2月11日 |
Study on the Rapid Plant Regeneration of Viola diffusa Ging.by Liquid Shake Culture |
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| DAI Rong-chun,HE Wen-jin,LIN Rong-hua,ZHU Jin-mao,CHEN You-qiang.Study on the Rapid Plant Regeneration of Viola diffusa Ging.by Liquid Shake Culture[J].Biotechnology,2003,13(4):25-26. |
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| Authors: | DAI Rong-chun HE Wen-jin LIN Rong-hua ZHU Jin-mao CHEN You-qiang |
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| Abstract: | The propagation of shoots of Viola diffusa Ging.can be promoted by liquid shake culture.Well-grown calli are induced from leaves in the media of MS+2,4-D 1.5+ZT 1.0.The calli are cultured in the solid media of MS+6-BA 2.0+NAA 0.5 for 14 days after liquid shake culture (90 r/min) in the liquid media of MS+6-BA 1.0+NAA 0.25 for 12 days,then the inducing rate of shoots is 97.5%.The plants can be transplanted after the shoots are cultured in the root media of MS+NAA 2.0+6-BA 0.25 for 1 month,and the living rate is over 90%. |
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| Keywords: | Viola diffusa Ging liquid shake culture plant regeneration |
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