| 枯草芽孢杆菌核黄素操纵子rib operon的克隆与表达 |
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| 引用本文： | 张西锋,王丽梅,刘梁,李万芬.枯草芽孢杆菌核黄素操纵子rib operon的克隆与表达[J].生物技术,2011,21(5). |
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| 作者姓名： | 张西锋 王丽梅 刘梁 李万芬 |
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| 作者单位： | 1. 武汉工业学院生物与制药工程学院,湖北武汉,430023
2. 武汉星辰现代生物工程有限公司,湖北武汉,430074 |
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| 基金项目： | 武汉工业学院引进(培养)人才科研启动资金项目 |
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| 摘 要： | 目的:构建产核黄素的枯草芽孢杆菌基因工程菌.方法:以穿梭载体pEB03构建核黄素操纵子的表达质粒载体pGJB13和pGJB14,与质粒pMX45分别转化产核黄素的枯草芽孢杆菌GJ07,并通过发酵摇瓶实验检测核黄素的产量.结果:得到产核黄素的工程菌GJ13 、GJ14和GJ08,在以蔗糖为碳源的发酵条件下,GJ08可产核黄素820mg/L,提高了约55%.结论:得到了产核黄素的高产菌种G J08.
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| 关 键 词： | 核黄素 枯草芽孢杆菌 操纵子 |
Cloning and Expression of Bacillus subtilis riboflavin operon |
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| ZHANG Xi-feng,WANG Li-mei,LIU Liang,LI Wan-fen.Cloning and Expression of Bacillus subtilis riboflavin operon[J].Biotechnology,2011,21(5). |
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| Authors: | ZHANG Xi-feng WANG Li-mei LIU Liang LI Wan-fen |
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| Institution: | ZHANG Xi-feng1,WANG Li-mei1,LIU Liang1,LI Wan-fen2(1.College of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,2.Wuhan Stars Modern Bio-engineering Co.Ltd,Wuhan 430074,China) |
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| Abstract: | Objective: Construction of engineering B.subtilis producing riboflavin.Method: riboflavin operon expression vector pGJB13 and pGJB14 were constructed using pEB03,a E.coli-B.subtilis shuttle vector.Plasmids pGJB13,pGJB14 and pMX45 were transformed into B.subtilis GJ07 to enhance riboflavin production using shake flask.Result: Got engineering B.subtilis GJ13,GJ14 and GJ08.With sucrose as carbon source,riboflavin production from GJ08 was about 820mg/L and 55% higher than that from the GJ07 in 60 hours.Conclusi... |
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| Keywords: | riboflavin Bacillus subtilis operon |
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