| 正交试验法优化半夏多糖的提取工艺 |
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| 引用本文： | 魏淑红.正交试验法优化半夏多糖的提取工艺[J].生物技术,2010,20(2):42-44. |
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| 作者姓名： | 魏淑红 |
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| 作者单位： | 西华师范大学生命科学学院,四川南充,637009 |
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| 摘 要： | 目的:建立半夏多糖的最佳提取工艺.方法:采刚水提醇沉法,以多糖提取率为指标,通过单因素试验和正交试验,确定半夏多糖的最住提取工艺条件.结果:最佳水提条件为料液比1:30,70℃提取1h,提取3次,最佳醇沉工艺条件为醇沉浓度70%,静置时间12h.在此条件下,半夏多糖的提取率为13.68%.结论:该工艺简便,重复性好,多糖的提取率高.
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| 关 键 词： | 半夏 多糖 提取工艺 单因素试验 正交试验 |
Study on Extraction Process for Polysaccharide of Pinellia ternata |
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| WEI Shu-hong.Study on Extraction Process for Polysaccharide of Pinellia ternata[J].Biotechnology,2010,20(2):42-44. |
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| Authors: | WEI Shu-hong |
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| Institution: | WEI Shu-hong(College of Life Science,China West Normal University,Nanchong 637009,China) |
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| Abstract: | Objective:To choose the optimum extraction technology of polysaccharide from the tuber of P.ternata.Method:The single fac-tor test and orthogonal experienment were employed for optimum extraction technology.Result:The best water extraction parameters are composed of m (P.ternata) : v (water) = 1: 30, three times(1 hour/time) under 70℃.The best alcohol precipitation conditions are adding ethanol till ψ(CH_3CH:OH) =70%, depositing for 12h.And the extracting rate is 13.68%.Conclusion:The method has charac-teristic of operating easily and reappearing well. |
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| Keywords: | P ternata extraction process orthogonal test mass fraction of polysaccharide |
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