PCR-based molecular markers for the fragrance gene in rice (Oryza sativa. L.) |
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| Authors: | S Garland L Lewin A Blakeney R Reinke R Henry |
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| Institution: | (1) Centre for Plant Conservation Genetics, Southern Cross University (SCU), Lismore, NSW, 2480, Australia+61-2 66222080, AU;(2) Yanco Agricultural Institute, NSW Agriculture, Yanco, NSW, 2703, Australia, AU;(3) Grains Processing, BRI Australia Ltd, North Ryde, NSW, 2113, Australia, AU |
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| Abstract: | The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that
was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based
marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped
near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles
from non-fragrance-producing alleles at the fgr locus.
Received: 19 October 1999 / Accepted: 16 December 1999 |
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| Keywords: | Fragrance Rice PCR marker Oryza sativa |
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