Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice |
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| Authors: | J H Jia D S Zhang C Y Li X P Qu S W Wang V Chamarerk H T Nguyen B Wang |
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| Institution: | (1) Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China e-mail:bwang@igtp.ac.cn Fax: 8610-64873428, CN;(2) Department of Plant and Soil Science, Texas Tech University, Lubbock TX 79409, USA, US;(3) Rice Research Institute, Tianjin Agricultural College, Tianjin 300381, China, CN |
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| Abstract: | TGMS (thermo-sensitive genic male-sterile) rice is widely used in hybrid rice production. Because of a specific temperature
requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered reverse thermo-sensitive genic
male-sterile line, J207S, has an opposite phynotype compared to the normal TGMS lines. J207S is completely sterile when the
temperature is lower than 31°C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility
of J207S was controlled by a single recessive gene which was first named as rtms1. An F2 population from the cross between J207S and E921 was developed and used for molecular mapping of the rtms1 gene. The AFLP (amplified fragment length polymorphism) technique, combined with BSA (bulked segregant analysis), was used
to screen markers linked to the target gene, and eight polymorphic AFLP loci were identified. Co-segregating analysis using
the F2 population showed that two of them, Rev1 and Rev7, were closely linked to the target gene with a recombinant rate of 3.8%
and 7.7%, respectively. Both Rev1 and Rev7 were found to be single-copy sequences through Southern analysis. Rev1 was subsequently
mapped on chromosome 10 with a doubled-haploid mapping populations derived from the cross CT9993 × IR62266 available at Texas
Tech University. RM222 and RG257 were linked to Rev1 at a distance of 11.8 cM and 4.6 cM, respectively. Additional SSR markers
from the rice map of Cornell University, RFLP markers from the map of RGP in Japan and the map of Texas Tech University were
selected from the region surrounding Rev1 on chromosome 10 to conduct the fine-mapping of the rtms1 gene. Presently, rtms1 was mapped between RM239 and RG257 with genetic distance of 3.6 cM and 4.0 cM, respectively. The most-closely linked AFLP
marker, Rev1, 4.2 cM from the rtms1 gene, was sequenced and converted into a SCAR (sequence characterized amplified region) marker which could facilitate marker-assisted
selection of the rtms1 gene.
Received: 2 November 2000 / Accepted: 21 November 2000 |
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| Keywords: | Rice Reverse TGMS gene AFLP MAS Molecular mapping |
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