AFLP-derived STS markers for the identification of sex in Asparagus officinalis L. |
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Authors: | S M Reamon-Büttner C Jung |
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Institution: | (1) Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany Fax: +49 (431) 880 2566, DE |
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Abstract: | For a simple, rapid and PCR-based screening of sex in the cultivated asparagus (Asparagus officinalis L.), we developed five STS markers from previously mapped, low-copy, sex-linked AFLP markers. A male/female PCR assay was
feasible with these STS markers either by direct amplification or by digestion with restriction enzymes. Similar to the AFLP
markers from which they were derived, STS4150.1, STS4150.2, STS4150.3 and STS3156 did not give recombinants in five different
populations. STS3660 could be scored codominantly, enabling the differentiation of XY from YY males in the screened F2 mapping population. The use of the sex-linked STS markers should allow early identification of sex, thus accelerating the
breeding process for new asparagus varieties. Further, 10 additional AFLP markers obtained with PstI/MseI primer combinations have been mapped on the L5 chromosome, bringing the total number of known AFLP and STS markers flanking
the sex locus to 24. These markers can be utilized for fine mapping of the sex gene in asparagus, which will pave the way
for a map-based cloning approach.
Received: 31 May 1999 / Accepted: 22 June 1999 |
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Keywords: | AFLP STS Sex-linked DNA markers PCR markers Asparagus officinalis L |
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