Identification of quantitative trait loci controlling developmental characteristics of Brassica oleracea L |
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Authors: | Sebastian R L Kearsey M J King G J |
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Institution: | (1) Plant Genetics and Cell Biology Group, School of Biological Sciences, The University of Birmingham, Birmingham, B15 2TT, UK, GB;(2) Plant Genetics and Biotechnology Department, HRI, Wellesbourne, Warwick CV35 9EF, UK e-mail: graham.king@hri.ac.uk, GB |
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Abstract: | A segregating population of F1-derived doubled haploid (DH) lines of Brassica oleracea was used to detect and locate QTLs controlling 27 morphological and developmental traits, including leaf, flowering, axillary
bud and stem characters. The population resulted from a cross between two very different B. oleracea crop types, an annual cauliflower and a biennial Brussels sprout. A principal component analysis (PCA), based on line means,
allowed all the traits to be grouped into distinct categories according to the first five Principal Components. These were:
leaf traits (PC1), flowering traits (PC2), axillary bud traits (PC3 and 5) and stem traits (PC4). Between zero and four putative
QTL were located per trait, which individually explained between 6% and 43% of the additive genetic variation, using the multiple-marker
regression approach to QTL mapping. For lamina width, bare petiole length and stem length two QTL with opposite effects were
detected on the same linkage groups. Intra- and inter-specific comparative mapping using RFLP markers identified a QTL on
linkage group O8 accounting for variation in vernalisation, which is probably synonymous with a QTL detected on linkage group
N19 of Brassica napus. In addition, a QTL for petiole length detected on O3 of this study appeared to be homologous to a QTL detected on another
B. oleracea genetic map (Camargo et al. 1995).
Received: 28 March 2001 / Accepted: 25 June 2001 |
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Keywords: | Brassica vegetables QTL Developmental traits Genetic analysis Linkage mapping |
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